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Tips to Make the Most of a Scientific Conference

FlipGFP, a novel fluorescence protease reporter to study apoptosis

It’s CRISPR Week at Addgene!

Single-cell tracking of lineage and identity with CellTag

A new platform for fast functional characterization of potentially cancerous mutations

What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

Bringing Sustainable Practices to the Lab: Easy Action Items

Visualizing Genomic Loci with CRISPR-Sirius

Science Communication for Everyone

Plasmids 101: Five Popular Model Organisms

GFP Fusion Proteins - Making the Right Connection

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

New and Upcoming Viral Vectors - Spring 2019

What's New in CRISPR - Spring 2019

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Improving Transduction Efficiency with Exosome AAVs

Designing Your Chalk Talk for the Academic Job Interview

Bringing Sustainable Practices to the Lab: Innovations

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Reproducibility for Everyone: Lessons from an Open Science Collaboration

Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

Targeted Mutagenesis with EvolvR

Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

Bringing Sustainable Practices to the Lab: Recycling

CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Lab to Office Culture Shock

The Breast Cancer Microenvironment: A Tumor’s Backstage Team

The Fluorescent Vegetables in Aptamer Soup

Tetbow: Bright Multicolor Labeling for Neuronal Tracing

Fluorescent Biosensors for Measuring Autophagic Flux

Supporting Reproducibility with a Connected ELN

2018 #DeckTheLab Contest Winners

Celebrating 15 Years of Scientific Sharing

Phage Directory: From Phage Therapy to a Repository of Phage Information

15 Years of Addgene: The Top 15 Plasmids

2019 Michael Davidson and Roger Tsien Commemorative Travel Awards

CRISPR 101: CRISPR-mediated Plant Base Editors

The CRISPR babies saga shows the need for action, not more delays

What's New in CRISPR - Winter 2018

Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

Early Career Researcher Toolbox: Social Media for Scientists

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Winter is here and it's time to #DeckTheLab

CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

Plasmids 101: Biotinylation

Academic vs. Industry Postdocs

Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

Tips for arabidopsis transformation

Tips for a 1st time AAV user (by a Rookie AAV user)

Plasmids 101: Simplify Cloning with in vivo Assembly

Golden Gate Assembly upgrades: More fragments, faster assembly, and higher fidelity

9 tips for a successful postdoctoral experience

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Is this the right place for me? 8 tactics for choosing a lab

Plasmids 101: Codon usage bias

Celebrating One Million Plasmids Shared!

Engaging with science and society at pgEd

Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

The time and cost required to make a plasmid

CRISPR 101: Ribonucleoprotein (RNP) delivery

Enabling high school research at the Journal of Emerging Investigators

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Finding nucleic acids with SHERLOCK and DETECTR

Microbes: Look, Listen, and Tell

When GFP lets you down

Plasmids 101: 5 factors to help you choose the right cloning method

RaPID Detection of RNA-protein Interactions

Neuronal labeling with Spaghetti Monster

Using AAV for neuronal tracing

Overcoming the Challenges of Lentiviral Production

Creating accessible biology activities in schools with BioBits

AAVs in retinal gene therapy

Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

Popular retroviral vectors and their uses in scientific research

Communicating your science with help from ComSciCon

"Build Your Plasmid" the game - Play to teach

10 Basic tips for mammalian cell culture

Replacing paper: tips for choosing an electronic lab notebook

Hassle-free 96-well format epitope tagging using Cas9 ribonucleoprotein

Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

BeHeard Award 2018: Diseases of glycosylation, arginine mutagenesis, & neural development

dTAG - You're it!

Using ultrasound to image bacteria in vivo: acoustic reporter genes

Adenoviral Vector Production and Troubleshooting

Why and how to start studying chromatin (with Addgene's help)

Plasmids 101: Protein Expression

CRISPR Cheat Sheet

Interview with Avital Bailen: iGEM and the OriginALS team

Rabies and Neuronal Tracing

Educational planarian tools for teaching developmental biology techniques

Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

FPbase: A new community-editable fluorescent protein database

Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

Plasmids 101: Repressible Promoters

Minisymposium on Reproducibility Livestream

Analyzing CRISPR Editing Results with ICE from Synthego

Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Split-BioID: An Improved Method for Studying Protein-Protein Interactions

Switch to GECO? An overview of AAV Encoded Calcium Sensors

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Transferable Skills Guide: External Collaborations

Delivery Methods for Generating iPSCs

Podcast: Interview with Addgene's Founders

Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

RANbodies: Reporter Nanobody Fusions

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Adeno-associated Viruses (AAVs) for Genome Editing

Lab Automation at Addgene

CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart

New Neuroscience Tool: The SF-iGluSnFr Glutamate Sensor

When Fidelity Matters: A frank discussion about ligase fidelity

The Importance of High Titer for AAV Transductions

Plasmids 101: Secondary Nanobody Toolbox

Learning Technical Skills at a Lab Central Workshop

New Videos: Addgene Lab Tips

CRISPR Challenges: Standardization and Homology Directed Repair

CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

Technologies Enabled by NanoLuc® Luciferase

Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

#Deckthelab Contest Winners 2017

Transferable Skills Guide: Managing a Team

In Vivo Biotinylation of Bacterial Fusion Proteins

PEI Calculator for Planning AAV Packaging Transfections

Plasmids 101: Inducible Promoters

Top Requested AAV of 2017: pmSyn1-EBFP-CRE

Visualizing Protein Turnover In Situ

Transferable Skills Guide: Identifying Your Transferable Skills

Top Requested Plasmid of 2017 - pMD2.G

15 Hot Plasmids from 2017

Identifying Sequence Elements with SnapGene's Feature Database

Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

The Strength of Story Telling

A Novice's Perspective on Neuroscience - SfN2017

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

New CRISPR Web Resources and #12DaysofCRISPR Recap

Hot Plasmids Episode 4: B. subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!

CAPTURE-ing Chromatin Interactions: Using CRISPR-dCas9 to Study Gene Regulation

Transferable Skills Guide: Time Management

CRISPR 101: RNA Editing with Cas13 and REPAIR

Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

Transferable Skills Guide: Teamwork

AAV Titers: Where do they come from and what do they mean?

Transferable Skills Guide: Career Planning Resources

Career Insights: Technical Support Specialist

Adeno Associated Virus (AAV) for Cell and Gene Therapy

Cultivating Community Science at BosLab

Oh, The Places You Can Go: Careers in Science Communication - Product Management

Light Up Your Experiments with the Michael Davidson Collection

Plasmids 101: NGS Quality Control for Pooled Libraries

Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!

With an Eye Towards the Future, We Look Back at the March for Science

Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Oh, The Places You Can Go: Careers in Science Communication - Product Development

3D Printing Meets CRISPR Cas9

Fluorescent Tagging of Endogenous Genes with SapTrap

A Deep Dive into BioBuilder

Fluorescence Microscopy Techniques - Which is Best for Me?

Tips for Working in Industry & Success in Management: Interview with Crystal Shih

Oh, The Places You Can Go: Careers in Science Communication - Science Writing

In Memoriam - The Origins of pBABE and Generations of Cancer Research

AAVs CREATed for Gene Delivery to the CNS and PNS

Science communication: 9 Strategies to Get Your Foot in the Door

Science Careers: Unruly Interests Feed Many Paths

CRISPR 101: Targeting RNA with Cas13a (C2c2)

Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

Some Like it Hot: Thermostable GeoCas9

AAV Vector Quality Control: Going the Extra Mile with NGS

Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

Cloning Mammalian Cells with the Agarose Method

Pushing the Limits of DNA Assembly

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Plasmids 101: Monitoring Cell Mobility Using Fluorescent Proteins

Human Germline Editing Using CRISPR

History of Fluorescent Proteins

Plasmids 101: An Inside Look at NGS Plasmid Quality Control

Visualizing Translation at the Single Molecule Level

Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

Lighting Up Cell Signaling with Photoswitchable Kinases

Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

Supporting Rare Disease Research with BeHeard 2017

Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

DIY DNA Ladders from Penn State University

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Custom CRISPR Screens & the Green Listed Software

Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

Improved Plasmid Maps Powered by SnapGene

Plasmids 101: Introduction to FRET

Who Gives a Tweet? 9 Facts About Scientists on Twitter

Plasmids 101: Visualizing Subcellular Structures & Organelles

How Dry Ice Affects Sample pH & How to Avoid It

New Educational Resource: The Addgene Videos Page

Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Important Considerations When Using AAVs

Choosing the B(right)est Fluorescent Protein: Photostability

Addgene's a Nonprofit? Nonprofit Awareness Day 2017

Beginner's Guide to Viral Vectors

A Practical Approach to Choosing the B(right)est Fluorescent Protein

Luminescent Imaging with Nano-lanterns

Anti-CRISPRs: Switching Off CRISPR-Cas9

Plasmids 101: Fluorescent Biosensors

New Podcast Segment: Hot Plasmids

Retrograde AAV: Making the Journey from Axon to Nucleus

Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Multiplex Genome Editing with CRISPR-Cpf1

Plasmids 101: Fluorescent Protein Timers

How to Design Your gRNA for CRISPR Genome Editing

Tips for Getting a Faculty Position

SciComm with the Experts at Science in the News Part 2

Plasmids 101: Photoactivatable Fluorescent Proteins

March for Science

Addgene Depositors Get More Citations

The Power Behind NGS Plasmid Validation: seqWell

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Plasmids 101: Aptamer Fluorophores

Plasmids for Endogenous Gene Tagging in Human Cells

Screening for Successful Genome Editing with Digital PCR

Plasmids 101: SunTag and Fluorescent Imaging

Addgene’s Viral Service - Why Virus? Why Now?

Quick Guide to All Things Lentivirus

SciComm with the Experts at Science in the News Part 1

Cancer and the Immune System: Deciphering the Relationship

What Do I Do Now? Academic v. Non-Academic Career Decisions

Quick New Way to Deposit Plasmids: The Deposit Spreadsheet

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Addgene Moves to NGS Verification Powered by seqWell

CRISPRainbow and Genome Visualization

Science Career Options

Deep Mutational Scanning with One Pot Saturation Mutagenesis

How to Write a Scientific Review Article

CRISPR 101: Epigenetics and Editing the Epigenome

5 Science Rap Videos & Their Creator: Tom McFadden

MXS Chaining

Plasmids 101: Environmental Plasmids

Plasmids: Simplified

Plasmids 101: Gateway Cloning

Your Top Requested Plasmid in 2016!

Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Truncated gRNAs for Regulating Gene Expression

Top Requested Lentivirus and AAV of 2016

Michael Davidson and Roger Tsien Commemorative Travel Awards

27 Hot Plasmids from 2016

Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Mesothelioma - Causes, Symptoms, and Treatment

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

With Our New Viral Service, We're Taking CRISPR Further!

'Tis the Season to #DeckTheLab

Plasmids 101: Knockout/Knock-In Plasmids

Bricking Science: Portraying Scientific Reality Through LEGO

Tips for Improving Your Next Manuscript

10 Great Guest Posts We're Thankful For

Using Addgene's New Viral Service for Your Research

Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

Antibiotic Resistance: An Old Solution but a New Problem

Plasmid Grant to Aid Cervical Cancer Screening in Ghana

Editor's Choice, October 2016

Using Video to Share Your Science: We Share Science

FastCloning: A Newer, Simpler Cloning Technique

Plasmids 101: TOPO Cloning

Changing Labor Laws Bring Increased Postdoc Wages

5 Reasons to Use Reddit for Science Communication

6 Tips for Grant Writing

Healthcare Consulting: A Door to the Business of Life Sciences

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Editor's Choice, September 2016

Grad School Advice Part 2: Building Community

Sequencing Options for CRISPR Genotyping

Technique: Probe Phage Genomes for Host Binding Proteins

Four Factors that Differentiate the Stem Cell Field

Tips for Writing a Good Cover Letter

5 Great Apps for Lab Life

Tips for Technical Support Calls

Running for Rare Disease, Running for FOP, Running for AJ

Multiple Plasmids at a Low Price: Kits from Addgene

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Addgene's Cancer Collection Pages Connect the Research Community

Editor's Choice, August 2016

Grad School Advice Part 1: Picking a Lab and a Project

Adapting Toehold Switches to Detect Zika Virus

Developing Lab Management Software for Biology

10 Steps to a Perfect Science Talk

Cas9 Activators: A Practical Guide

Single Base Editing with CRISPR

5 Tips for Troubleshooting Viral Transductions

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Editor's Choice, July 2016

Google Forums Round Up: First Impressions of NgAgo

Site Directed Mutagenesis by PCR

Recombinase-based State Machines Enable Order-dependent Logic in vivo

AAV: A Versatile Viral Tool for Gene Expression in Mammals

Gendered Innovations: Why Does Sex of the Cell Matter?

Special Delivery: Fluorophore Targeting for FRET Studies

Cpf1 Update: Comparison to Cas9 and NgAgo

Generating Mouse Models Using CRISPR/Cas9

Addgene at the ASM Microbe 2016 Conference

Plasmids 101: Methylation and Restriction Enzymes

Tips for CRISPR Gene Editing in Mice

Using Phosphoserine to Study Protein Phosphorylation

Evolution of Lab Techniques

Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

Introducing Addgene's Microbiology Pages

Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Using Virus in Your Research - A Primer for Beginners

How to Keep a Lab Notebook for Bioinformatic Analyses

PiggyBac-ing Through the Genome Editing Field

Plasmids 101: Optimizing Plasmid Yields

Podcast: A Malawian Professor's Path to Biotech Research

Lentiviral Vector Uses and Overview

Searchable and Sortable gRNAs for Your Next CRISPR Experiment

Plasmids 101: Colony PCR

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

The Challenges of Cell Culture

CRISPR Antimicrobials

Plasmids 101: FLEx Vectors

Casilio: An Adaptive, Multitasking “CRISPR-OS”

Genome engineering using Cas9/gRNA Ribonucleoproteins (RNPs)

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

R Bodies: Membrane-Rupturing Microscopic Tools

Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

Getting the Most from Your Lentiviral Transduction

Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

Plasmids 101: Terminators and PolyA signals

Plasmid Cloning by PCR

Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Modulate the Activity of 17 Signaling Pathways with One Kit!

Minigenomes - a Safe Way to Study Dangerous Viruses

Suggest A Plasmid You'd Like to Find at Addgene

Tips for Titering Your Lentiviral Preps

Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

Optogenetics + CRISPR, Using Light to Control Genome Editing

CRISPR Methods for Bacterial Genome Engineering

Call for Guest Bloggers

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Experimenting with New Careers while in Grad School

PITChing MMEJ as an Alternative Route for Gene Editing

CRISPR-Cas9: Tips for Optimizing sgRNA Activity

Plasmids 101: Restriction Cloning

REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

How to Lead a Great Meeting

Anatomy of a Plasmid Page at Addgene

Components of CRISPR/Cas9

CRISPR 101: Multiplex expression of gRNAs

Treating Muscular Dystrophy with CRISPR Gene Editing

Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Addgene's Tips for Plasmid Quality Control

Finding Your Perfect Job After University

How to Deposit Your Plasmids with Addgene

Your Top Requested Plasmid in 2015!

28 Hot Plasmid Technologies from 2015

Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

A Better Way to Get Customer Support: The Help Center

An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

Addgene's Top 10 Blog Posts of 2015

Plasmids 101: Stringent Regulation of Replication

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

#DeckTheLab to Win a Tasty Winter Treat This Holiday Season

Thoughts on the Future of Research 2015 Boston Symposium

Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Illuminating Epigenetics with A FRET Based Biosensor

Networking and Inspiring Young Scientists at Freshers Fairs

MassBioEd Career Exploration Day at Addgene

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Inntags: Innovative Protein Epitope Tagging

Building Global Connections with the International Mentorship Program USA-EUROPE

The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Tips for Screening with Yeast Two Hybrid Systems

The Future of Research Symposium Boston 2015

Cpf1: A New Tool for CRISPR Genome Editing

Tips from the Repository Trenches: Using Barcodes to Track Samples

Important Considerations in Optogenetics Behavioral Experiments

pSiM24: Simplifying Plant Genetic Engineering

CRISPR 101: Mammalian Expression Systems and Delivery Methods

New Tool for Lineage Tracing: The ClonTracer Library

The Materials Science of Optogenetics Experiments

Donations from Addgene to Yield Answers for Rare Disease Researchers

A Primer on Optogenetics: Introduction and Opsin Delivery

Synthetic Photobiology: Optogenetics for E. coli

The Importance of a Fun Workplace: Company Culture at Addgene

How to Negotiate a Successful Lab Start Up Budget

Plasmids 101: Golden Gate Cloning

Don’t Wait To Launch Your Health Science Startup

A Career in Grants: How To Become a Grant Professional

Genome-wide Screening Using CRISPR

Addgene Welcomes John Doench to the Advisory Board

Mapping the 4D nucleome with CRISPR/Cas9

Gaining Leadership Skills Volunteering at a Professional Organization

Seeing Red: Simple GFP Photoconversion

CRISPR 101: Validating Your Genome Edit

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Experimenting in an Open Source lab: from CRISPR to Cats

IDP and your PI: A Roadmap for Career Planning and Personal Development

Light Sheet Fluorescence Microscopy

A Match Made in Heaven: CRISPR and AAV

6 Steps to Submitting a Resume That Gets Seen

Even more elegant: Single injection CRISPR/Cas9 in C. elegans

Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

Sleeping Beauty Awakens for Genome Engineering

Another Pathway into Cells: iTOP

Easing the Protein Purification Process with pCri

Science Communication Snapshot: DayCon 2015

More Data for You: Find Articles Citing Addgene Plasmids

Plasmids 101: Blue-white Screening

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

Online Social Networking for Scientists

Cre-ating New Methods for Site-specific Recombination in Drosophila

Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

CRISPR 101: Non-Homologous End Joining

Plasmids 101: Control Plasmids

Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

Management for Scientists: Managing vs. Leading

500,000 Plasmids Shipped and Counting!

CRISPR 101: Homology Directed Repair

The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

CRISPR 101: A New Series on Genome Editing & CRISPR-Cas

Science as Art

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Management for Scientists: Delegating is Key

CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

Plasmids 101: E. coli Strains for Protein Expression

Management for Scientists: Giving Feedback

Trends in CRISPR and SynBio Technologies [Slideshare]

Career Coaching for Scientists: Why and Where Do I Find One?

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

Plasmids 101: Cre-lox

Management for Scientists: Seeking Feedback

22 Hot Plasmid Technologies from 2014

Resume Writing for Non-academic Science Careers

Addgene’s Top 10 Blog Posts from 2014

Plasmids 101: Protein tags

Management for Scientists: What Makes a Good Manager Anyway?

Make a Splash: Notions of Scientific Impact Are Evolving

Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

How Exercise Purges Stress and Keeps Depression at Bay

We've Launched Addgene's New & Improved Website!

Fueled by Coffee at #SfN14

Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

Plasmids 101: Common Lab E. coli Strains

Tips for Using FRET in Your Experiments

Countdown to Halloween @Addgene

How-to: 5 Steps to a Great Panel Discussion

A Scientist's Guide to Ebola

Choosing Your Fluorescent Proteins for Multi-Color Imaging

Future of Research Conference - Remarkable Opening Session

ICYMI: Addgene’s Plasmids 101 eBook

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

Plasmids 101: Multicistronic Vectors

A Conference By Postdocs For Postdocs: Future of Research

Plasmids 101: How to Verify Your Plasmid

Advice for Moving Into Sales After Your Science Postdoc

Choosing Your Perfect Empty Backbone

SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

How to Make Friends and Meet People at a Scientific Conference

Data Freedom: The Expansion of Data Sharing in Research Publications

The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

3 Challenges in Plant Synthetic Biology

Plasmids 101: Viral Vector Elements

Working with Nuclear Receptors

Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Synthetic Biology & the Importance of Assembly Standards

Inside Addgene's 10 Year Anniversary Gala

Scientist Networking: What is an Informational Interview?

6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

Plasmids 101: Luciferase

Designer PUF Proteins for Any RNA Target

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

London Calling - Addgene’s New Europe Office in London

Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Developing Transferable Skills During Science Training

Tips for Using BLAST to Verify Plasmids

Dos & Don'ts When Publishing a Scientific Manuscript

Which Fluorescent Protein Should I Use?

Plasmids 101: Green Fluorescent Protein (GFP)

10 Ways to Share Your Science!

Celebrating Accomplishments in the Lab

Hot Plasmids: FRET-Based Biosensors

Why Do I Need an MTA Anyway?

Advice for Scientists Starting a Lab

Your Lentiviral Plasmid FAQs Answered

Advice for Choosing a Research Project

The Stingy Scientist: How the Baby Gel Box Was Born

Finding and Joining Your Dream Lab

Plasmids 101: The Promoter Region – Let's Go!

9 Tips to Achieve Success in Academia

In Living Color: The Skinny on In Vivo Imaging Tools

Plasmids 101: Mammalian Vectors

Negotiating Work and Life: How to Find the Joy

CRISPR-Cas9 FAQs Answered!

Keeping Up With CRISPR/Cas9

Mentoring for Scientists: I Have a Mentor, What Now?

Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

Why Scientists Should Give Twitter a Try

Plasmids 101: Yeast Vectors

Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

5 Steps to Writing Grant Proposals for Grad Students

Plasmids 101: Origin of Replication

Will You Be My Mentor? Finding and Asking for Mentoring Support

Plasmids 101: Antibiotic Resistance Genes

Save Time and Money by Making Your Own Competent Cells

Quick, Versatile Plant Transgenesis with GreenGate Plasmids

Extracurricular Activities for a Strong Science Career Path

Choosing a Good Mentor for Scientists

Plasmids 101: What is a plasmid?

The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

Using CRISPR/Cas9 to Edit Disease Out of the Genome

Three Tips to Organize Your Lab Notebooks in the New Year

The Addgene Zoo - A Plasmid Repository with a Diverse Collection of Genes

Top 10 Open Science Developments of 2013

"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Kiran Musunuru on the Newest TALEN Genome-Editing System

Overwhelmed? Take a Break With Our 5 Favorite Science Comics

From our Table to Yours: An Inside Look at Lunch at Addgene

History of CRISPR Cas - A tale of survival and evolution

Let There Be LITE Plasmids

Tag Your Favorite Yeast Genes with Ease

Addgene’s New Way to Help You Share Science

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