Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Over 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database. Previously developed genome editing methods using nucleases and base editors have the potential to correct only a minority of those variants in most cell types. But ...
Each plasmid in Addgene’s collection contains a story, hidden in the wealth of data all plasmids come with. The story tells who made the plasmid, who is using it, where it comes from and where it’s going, what it does, and even what advancements it has helped make.
As co-founder Melina Fan, PhD, transitions to Chief Scientific Advisor, we reflect on the problem she set out to solve 20 years ago, the community she helped build, and where she and Addgene go next.
Modeling is not just a job for humans — it is also a job for many different organisms. And like human modeling, biological modeling turns out to be surprisingly glamorous and diverse. But what comes to mind when you picture a “model organism”? A mouse? A pesky fruit fly? ...
For many PhDs, the transition from graduate school or a postdoc into the next career step can feel like running an experiment without a clear protocol. You may have spent years optimizing conditions, troubleshooting assays, and becoming deeply familiar with one narrow scientific ...
Guiding a cell through differentiation to its eventual fate requires a precise recipe of molecular signals, with transcription factors (TFs) as the key ingredients. With the right combination of TFs, scientists can also reprogram cell identity in the lab. In immune cells, such ...
iGEM isn’t any old science fair. “The world’s biggest synthetic biology competition” brings together hundreds of student teams to build projects and solve problems using molecular biology.
Every few months we highlight some of the new plasmids, antibodies, viral preps, and other materials in the repository through our Hot Plasmids articles. This month, we’ve got some new biosensors for neuroscience research, popular plasmids for a common lab enzyme, and more!
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