By Emily P. Bentley
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In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. CGBEs first convert a cytosine (C) to uracil (U), just like Cytosine Base Editors (CBEs). But unlike CBEs, CGBEs then excise the U to create an abasic (empty) DNA site using ...
The first base editors revolutionized CRISPR gene editing. Cytosine base editors (CBEs) and adenine base editors (ABEs) chemically modify target bases without breaking the DNA backbone, making them efficient and precise tools for altering DNA sequences. These first base editors ...
What’s in a type? That which we call CRISPR, by any other name would…probably still edit genomes.
Every few months, we highlight some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.
Twenty years of accelerating scientific discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens when scientists come together ...
Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair (HDR), the pathway for resolving DNA double-strand breaks (DSBs) preferred by researchers. Compared to non-homologous end joining (NHEJ), HDR occurs at a relatively low ...
The versatility of CRISPR allows you to play with DNA in a number of ways, from small edits that change single base pairs, to chromosomal inversions and large deletions. Many of these methods rely on Cas9 or a derivative of Cas9, but the ever-expanding repertoire of CRISPR has ...
