Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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ByBalázs Csoma, Hungarian Research Network CRISPR nucleases are remarkably precise molecular tools for cutting DNA. But “remarkably precise” does not mean “perfectly specific.” In reality, CRISPR nucleases occasionally make mistakes and cleave DNA sequences that only resemble ...
Deaminet 2026, held in Palm Springs in late January, brought together researchers studying deaminase enzymes across disciplines: from structural biologists resolving APOBEC proteins at atomic resolution, to cancer biologists dissecting mutational processes, to medical ...
Do you have a green thumb — or, perhaps, are you one of those people who kills every plant they touch? The whole trouble with houseplants is they can’t bark, meow, or cry when they need something. But what if we could equip plants with a way to warn us about concerning growing ...
Every few months we highlight some of the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles. This month, check out hot new AAV packaging plasmids, CRISPR libraries, and more!
As 2025 closed, we received a request that perfectly embodies why Addgene exists: a researcher in Laos needed a unique set of plasmids to advance their research. Thanks to five different labs across the U.S. who deposited their plasmids with Addgene to share with the rest of the ...
The genome-editing tool CRISPR is famously RNA-guided... except when it’s not. Turns out, carefully designed RNA-DNA hybrid strands work just as well—or maybe even better—at guiding Cas nucleases to specific genomic targets.
Throughout its 20-year history, Addgene’s mission has been to empower scientists to advance discoveries by reducing barriers, facilitating access to high-quality research materials, tools, and resources, while also fostering scientific collaboration. The first request through ...
Who doesn’t love a library? A traditional library might contain thousands or millions of different books. Addgene’s pooled libraries are a little different: each is a collection of plasmids that share the same backbone and differ only in a small region. This makes them useful ...
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