Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
Subscribe
Taxonomy tells us that technically, we are all fish — which is one of the reasons zebrafish are such useful model organisms. Their conveniently transparent embryos and fast maturation time have taught us about vertebrate development, neuroscience, drug development, and more.
This post was written by Angelo Nicolaci, a PhD student at Moffitt Cancer Center.
Few lab routines are as commonplace — or as patience-testing — as DNA preps. For most labs, getting plasmid DNA still means streaking plates, growing an overnight culture, performing a miniprep, and checking concentration and quality. Researchers can spend a few days (or ...
In July, Addgene hosted a webinar titled “CRISPR Pooled Libraries: Optimizing Knockout Library Design.” If you missed it, don’t worry! Not only is it still available to watch, but this post covers the same material.
Every few months we highlight some of the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles. This month, we’ve got lots of exciting materials to talk about, from the latest prime editors and new CRISPR tools to antibodies and a ...
Every August, the CRISPR community returns to Cold Spring Harbor Laboratory for the Genome Engineering: CRISPR Frontiers conference. This year’s meeting gathered researchers across the field, from the bacteriophage-defense biology that revealed these systems to clinical research ...
If you came to Addgene looking for a plasmid sequence and ran into a login request instead, we understand why that’s frustrating. You knew exactly what you needed, and now there’s one more step between you and the information you’re looking for. We didn’t add this step to slow ...
Over 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database. Previously developed genome editing methods using nucleases and base editors have the potential to correct only a minority of those variants in most cell types. But ...
