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New and Upcoming Viral Vectors - Spring 2019

Posted by Leila Haery on Mar 26, 2019 9:40:05 AM

Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released so far in 2019. You can also browse our entire AAV inventory!

Our new AAVs include:

  • pGP-AAV-syn-FLEX-jGCaMP7b-WPRE
  • pAAV.hSyn-FLEX.iGABASnFR
  • pAAV.hSyn-FLEX.iGABASnFR.F102G
  • pAAV-Syn-Archon1-KGC-GFP-ER2
  • pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]
  • pAAV-FLEX-tdTomato
  • pAAV-GFAP104-mCherry
  • pAAV-mDlx-NLS-mRuby2
  • CAG-NLS-GFP
  • pAAV-hSyn-mCherry
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Topics: Viral Vectors

Improving Transduction Efficiency with Exosome AAVs

Posted by Klaus Wanisch on Mar 14, 2019 9:54:29 AM

Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often describe a process that begins with cell lysis to release AAV particles with subsequent purification steps. It’s also possible for AAV vectors to be spontaneously released from the producer cells into the supernatant during the production phase. However, this has not been well characterized as it is not a common event.

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Topics: Viral Vectors

A Media Supplementation Strategy to Improve AAV Yields

Posted by Meghan Rego on Dec 13, 2018 8:14:12 AM

By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs independent research? Since launching the viral service in 2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing novel methods for data analysis, our team’s goal is to provide scientists with new tools and protocols that they can easily incorporate into their own labs. This week we are pleased to announce the submission of the results of our first study which can be accessed here at bioRxiv.

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Topics: Viral Vectors

Tips for a 1st time AAV user (by a Rookie AAV user)

Posted by Beth Kenkel on Oct 23, 2018 7:49:25 AM

My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, I had never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools, I found myself writing AAV production tricks into the margins of my protocol. While these tips weren’t critical to the experiment, they definitely made my life easier!  In this post, I’ll share some AAV production, purification, and titration tips, while also summarizing the basic steps and analyses needed for packaging AAV.

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Topics: Techniques, Viral Vectors

Neuronal labeling with Spaghetti Monster

Posted by Benoit Giquel on Aug 14, 2018 8:42:01 AM

The central nervous system (CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These functions and others are controlled by collections of neurons that are intricately wired into circuits through synaptic connections (Shepherd, 2004). Understanding the structure and function of these neural circuits is essential for neuroscience research. The use of genetic tools for visualizing and perturbing circuits together with the development of methodologies to deliver genes to the CNS have recently made it much easier to map these neuronal networks.

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Topics: Viral Vectors

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