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New and Upcoming Viral Vectors - June 2019

Posted by Leila Haery on Jun 18, 2019 9:15:20 AM

Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released in the last few months. You can also browse our entire AAV inventory.

Our new AAVs include:

  • Control AAVs for serotype testing
  • jGCaMP7c for calcium detection
  • PSAM4 GlyR for chemogenetic inhibition
  • Flp expression for intersectional studies
  • And more!
Read More >

Topics: Viral Vectors

Addgene Protocols Go Viral: AAV Purification and Titration

Posted by Eddy Page on Jun 13, 2019 8:19:13 AM

Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to scientists around the world. In the years since, our scientists have optimized many steps in the viral production and quality control process to improve both the yield and quality of the viral preps. For example, last year we published an article describing a sucrose supplementation strategy to improve yield. In addition, as described in this blog post, the team developed a viral genome sequencing strategy to confirm the identity and purity of all AAV viral lots.

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Topics: Viral Vectors

Single-cell tracking of lineage and identity with CellTag

Posted by Beth Kenkel on May 9, 2019 9:15:18 AM

Direct reprogramming describes the process where differentiated cells are turned into a cell type of choice while bypassing the intermediate pluripotent state. Though a valuable tool for regenerative medicine, direct reprogramming is an inefficient process, with the majority of cells failing to develop the desired identity.

The development of single cell technologies, such as single cell RNA-seq (scRNA-seq), now allows scientists to identify the gene expression patterns unique to the few cells that successfully reprogram. Unfortunately scRNA-seq only captures the gene expression of cells at a single time point, making it difficult to investigate how early expression patterns influence reprogramming outcomes.
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Topics: Viral Vectors

New and Upcoming Viral Vectors - Spring 2019

Posted by Leila Haery on Mar 26, 2019 9:40:05 AM

Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released so far in 2019. You can also browse our entire AAV inventory!

Our new AAVs include:

  • pGP-AAV-syn-FLEX-jGCaMP7b-WPRE
  • pAAV.hSyn-FLEX.iGABASnFR
  • pAAV.hSyn-FLEX.iGABASnFR.F102G
  • pAAV-Syn-Archon1-KGC-GFP-ER2
  • pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]
  • pAAV-FLEX-tdTomato
  • pAAV-GFAP104-mCherry
  • pAAV-mDlx-NLS-mRuby2
  • CAG-NLS-GFP
  • pAAV-hSyn-mCherry
Read More >

Topics: Viral Vectors

Improving Transduction Efficiency with Exosome AAVs

Posted by Klaus Wanisch on Mar 14, 2019 9:54:29 AM

Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often describe a process that begins with cell lysis to release AAV particles with subsequent purification steps. It’s also possible for AAV vectors to be spontaneously released from the producer cells into the supernatant during the production phase. However, this has not been well characterized as it is not a common event.

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Topics: Viral Vectors

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