Viral Vectors 101: Producing Your rAAV

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AAV infection of retinal neurons.
Cartoon depicting factors to consider for a pooled library when creating an amplification protocol; details are in text of link.
 A viral particle enters a host cell, enters the host nucleus, is transcribed into RNA, and is translated into protein.
Cartoon schematic: a barcoded transfer plasmid and packaging plasmids are put in barcoded LentiX-293T cells. From this, an arrow points to virus particles with a barcode; an arrow from the barcode points to
Structure of a recombinant AAV vector, where two ITRs flank the gene cassette.
Cartoon depicting (a)

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