Viral Vectors

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A flowchart reflecting a cell fate decision tree. At each node, a transcription factor leads to two different outcomes depending on high or low expression. Each arrow is annotated with downstream genes. By following arrows through 3-5 nodes, various cell fates are decided: Non-reprogrammed fibroblast, MΦ/fibroblast, DC, monocyte/DC, MΦ/monocyte, lymphoid (NK), or mReg DC.
A cartoon depiction of a lentiviral genome forming an episome in the presence of non-functional LV integrase. The linear lentiviral genome has a long terminal repeat (LTR) on each end. A split arrow shows two paths for circular episome formation. Homologous recombination within the LTRs results in a single LTR in the circular episome. Non-homologous end joining results in two adjacent LTRs in the circular episome.
Graphic showing the workflow of using a pooled AAV CRISPR library in vitro. Step A shows the AAV containing the library with either the gRNA only or gRNA plus Cas9 and infecting cells expressing Cas9 or wild-type. The different guides are represented by different colors in individual cells. Step B shows the results of the positive or negative selection, with only one type of guide (color) being chosen. Step C shows verification using next generation sequencing.
AAV infection of retinal neurons.
Schematic depicting HEK-293T cells in a culture dish and Sf9 cells in a culture flask being used to produce various viruses
A graphic showing the request to shipping process for Addgene's AAV Packaged on Request service.

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