New Viral Vectors - Spring 2025

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Graphic showing the workflow of using a pooled AAV CRISPR library in vitro. Step A shows the AAV containing the library with either the gRNA only or gRNA plus Cas9 and infecting cells expressing Cas9 or wild-type. The different guides are represented by different colors in individual cells. Step B shows the results of the positive or negative selection, with only one type of guide (color) being chosen. Step C shows verification using next generation sequencing.
AAV infection of retinal neurons.
Schematic depicting HEK-293T cells in a culture dish and Sf9 cells in a culture flask being used to produce various viruses
A graphic showing the request to shipping process for Addgene's AAV Packaged on Request service.
AAV infection of retinal neurons.
A line and box diagram representing the genome, with relevant genes represented by labeled boxes, of gamma-retrovirus with five gene represented by boxes,

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