Viral Vectors 101: Parts of the AAV Packaging Plasmid

By Beth Kenkel

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Graphic of channelrhodopsin activated with blue light. Without light, the channelrhodopsin is inactive, no ions flow in, and neurons don't fire. With blue light, the channelrhodopsin opens and allows an influx of ions into the neuron. This results in neuronal firing.
An AAV with the viral genome inside. This zooms into the the AAV viral genome that is labeled. The viral genome is 4.4 kb maximum, and includes an ITR, promoter, and trangene. Option elements are LoxP sites, WPRE, and pA.

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