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Tips for a 1st time AAV user (by a Rookie AAV user)

Posted by Beth Kenkel on Oct 23, 2018 7:49:25 AM

My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, I had never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools, I found myself writing AAV production tricks into the margins of my protocol. While these tips weren’t critical to the experiment, they definitely made my life easier!  In this post, I’ll share some AAV production, purification, and titration tips, while also summarizing the basic steps and analyses needed for packaging AAV.

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Topics: Viral Vectors, Viral Vectors 101, Viral Vector Protocols and Tips, AAV

AAVs in Retinal Gene Therapy

Posted by Karen Guerin on Jul 31, 2018 8:55:07 AM

It was by serendipity that I got into the field of gene therapy, more specifically AAV-based retinal gene therapy. The year was 2001 and I started a job as a technician in a lab using adeno-associated viral vectors (AAVs) to treat an inherited retinal degenerative disease called Retinitis Pigmentosa. I quickly became fascinated by this emerging technology and its potential for the treatment of some genetic diseases.

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Topics: Viral Vectors, AAV

Switch to GECO? An Overview of AAV Encoded Calcium Sensors

Posted by Leila Haery on Apr 26, 2018 9:24:32 AM

As part of our partnership with the Penn Vector Core, we will be expanding our inventory of tools for calcium sensing. In this post, we’ll review the main categories of sensors we’ll have available.

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Topics: Viral Vectors, Neuroscience Biosensors, AAV, Neuroscience

Adeno-associated Viruses (AAVs) for Genome Editing

Posted by Tyler Ford on Mar 27, 2018 9:32:43 AM

Guest blogger Todd Waldman, Professor at Georgetown University, contributed to this post.

Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene expression and are particularly useful for gene delivery to the nervous system. For many years they have also been used to enhance the efficiency of genome editing. In this post we'll walk through a variety of ways you can use AAVs to improve your genome editing experiments (with and without targeted nucleases).

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Topics: Viral Vectors, AAV, Other Viral Vector Tools

The Importance of High Titer for AAV Transductions

Posted by Luke Hanley on Mar 1, 2018 9:11:54 AM

Many of us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells, titer, the amount of virus used, really does matter. (*psst*, quality definitely also matters).

Check out this post for a refresher on AAV titers

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Topics: Viral Vectors, Viral Vectors 101, Viral Vector Protocols and Tips, AAV

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