A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

By Jason Nasse

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The RepCap plasmid consists of three promoters within the rep coding region that encodes four different Rep proteins. This region also encodes three different VP proteins, the MAAP protein, and the AAP protein.
An AAV with the viral genome inside. This zooms into the the AAV viral genome that is labeled. The viral genome is 4.4 kb maximum, and includes an ITR, promoter, and trangene. Option elements are LoxP sites, WPRE, and pA.
anterograde retrograde tracing

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