Your next cool experiment requires some AAV. Where do you start? Plasmids of course! You just need three plasmids to start making the AAVs you need for your experiment:
- the packaging plasmid which contains the AAV structural and packaging genes,
- the adenoviral helper plasmid which contains the proteins needed for the virus to replicate,
- and the transfer plasmid which contains the viral genome.
In today’s blog post, we’ll focus on the packaging plasmid and take a look at each of its parts.