Studying proteins in their natural context is one of the biggest challenges in biology. From tumor suppressors to growth factors, some of the most clinically-relevant proteins are also the hardest to study. One common strategy is protein overexpression — boosting levels so ...
For most people, the most familiar source of bioluminescence is the firefly. Along with charming nighttime displays, these insects have contributed an important tool to the scientific community: luciferases, which have become common genetic reporters and alternatives to ...
This post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself or you could ...
Fluorescent imaging techniques have become indispensable tools for molecular and cell biologists over the last two decades, but their use can be limited by a few caveats. Since fluorescent proteins (FP) require external light activation, you can’t use fluorescence to monitor ...
