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HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

Posted by Alyssa Cecchetelli on Oct 10, 2019 8:41:58 AM

Part stable scaffold, part epitope binding region, all fused to a fluorescent protein. “Frankenbody,” like the infamous frankenstein, was constructed by grafting different parts together. By combining two optimized elements of an antibody probe, scientists can now more easily visualize single molecules and newly formed proteins. 

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Topics: Fluorescent Proteins, Localization with Fluorescent Proteins

Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

Posted by Jennifer Tsang on Jun 4, 2019 9:02:29 AM

Fluorescent proteins have enabled scientists to pursue creative research avenues previously unavailable to them. With these tools it’s now easy to monitor protein expression, localization, and protein-protein interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent proteins everyday. 

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Topics: Fluorescent Proteins

FlipGFP, a novel fluorescence protease reporter to study apoptosis

Posted by Alyssa Cecchetelli on May 21, 2019 8:10:19 AM

Apoptosis or “programmed cell death" plays a pivotal role in an array of biological processes including development, the immune system, and cell turnover. Apoptosis is a highly controlled process that is triggered by internal and external signals such as developmental cues and DNA damage. These signals activate a cascade of caspases, protease enzymes that cleave proteins. Executioner caspases are activated last in the cascade and are responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and death (Elmore, 2007).

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Topics: Fluorescent Proteins

Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

Posted by Jennifer Tsang on Apr 25, 2019 11:01:12 AM

We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen. This fluorescent protein joins mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and what can they be used for? Let’s take a look!

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Topics: Fluorescent Proteins

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Posted by Guest Blogger on Apr 9, 2019 9:13:55 AM

This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced Microscopy (University of Amsterdam).

Tagging a protein of interest with a fluorescent protein to study its function is one of the most popular applications of fluorescent proteins. These fusion proteins enable the observation of proteins in living cells and organisms. Both components of the chimera are encoded by DNA. Since researchers can generate almost any DNA sequence in the way that they like, the design and engineering of fusion proteins is relatively straightforward. However, generating a fusion while keeping all of the native properties of the protein of interest can be challenging. In this blog I discuss strategies to generate fusion proteins and highlight some aspects of their design. 

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Topics: Fluorescent Proteins, Fluorescent Proteins 101

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