Live and Let Dye: Self-Labeling Protein Tags

By Mike Lacy

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A protein ribbon model of nLightG and nLightR (a) and microscopy images showing expression of nLightG in cultures with NE and not in cultures without NE. The same experiments are repeated with nLightR, but there is faint expression in the cultures w/o NE.
Schematic showing MTRIA biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled receptors
Micrographs of biosensors expressed in human cell lines
flow cytometry overview image showing labeling and characterization of cells through the flow cytometer

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