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FPbase: A new community-editable fluorescent protein database

Posted by Guest Blogger on May 16, 2018 9:00:00 AM

This post was contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School.

The need for a community fluorescent protein database

As recognized by the 2008 Nobel Prize, fluorescent proteins (FPs) have become one of the most indispensable tools in modern biological research.  Any microscopist will tell you that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  The choice is non-trivial, however, as FPs are tremendously complicated entities with a large range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which are dramatically affected by environmental conditions (such as temperature, pH, fusion protein, etc...).  There are many online guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of the primary data one might require when making such a decision remains spread across literature in publications that introduce these tools.

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Topics: Scientific Sharing, Fluorescent Proteins

Switch to GECO? An overview of AAV Encoded Calcium Sensors

Posted by Leila Haery on Apr 26, 2018 9:24:32 AM

As part of our partnership with the Penn Vector Core, we will be expanding our inventory of tools for calcium sensing. In this post, we’ll review the main categories of sensors we’ll have available.

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Topics: Fluorescent Proteins, Viral Vectors

Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart

Posted by Tyler Ford on Mar 9, 2018 9:00:00 AM

Today on the Addgene Podcast, we have another interview conducted by European Outreach Scientist Benoit Giquel. Ben recently spoke with Joachim Goεdhart, a professor at the University of Amsterdam. In addition to creating and sharing many great fluorescent protein tools, Professor Goedhart is very active on Twitter and has helped us update many of our educational resources. Listen to hear all about Professor Goedhart, his lab, and some of the tools he’s developed.

 

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Topics: Fluorescent Proteins, Podcast

New Neuroscience Tool: The SF-iGluSnFr Glutamate Sensor

Posted by Tyler Ford on Mar 8, 2018 9:00:00 AM

In a previous blog post we discussed how fluorescent proteins can be used to construct biosensors, biological tools that monitor processes or detect molecules. Here we’ll be diving into the details surrounding SF-iGluSnFr, a recently upgraded biosensor designed to detect glutamate.

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Topics: Hot Plasmids, Fluorescent Proteins

Technologies Enabled by NanoLuc® Luciferase

Posted by Guest Blogger on Feb 8, 2018 7:17:02 AM

This post was contributed by Kyle Hooper at Promega.

Researchers have been sharing plasmids ever since there were plasmids to share. Back when I was in the lab, if you read a paper and saw an interesting construct you wished to use, you could either make it yourself or you could “clone by phone”. One of my professors was excellent at phone cloning with labs around the world and had specific strategies and tactics for getting the plasmids he wanted. Addgene makes it so much easier to share your constructs from lab to lab. Promega supports the Addgene mission statement: Accelerate research and discovery by improving access to useful research materials and information. Many of our technology platforms like HaloTag® Fusion Protein, codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the repository. We encourage people to go to Addgene to get new innovative tools. Afterall, isn’t science better when we share?

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Topics: Fluorescent Proteins

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