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Selectable transgene markers for plant transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings or callus (undifferentiated plant cell culture), ...
This post was contributed by guest blogger, Robert Orr, who recently received a Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute. What is RNAi? The loss-of-function (LOF) experiment functions as the building block of our understanding of complex biological ...
This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with plants doesn’t always have to be a time-consuming process. While developing transgenic hairy root lines in tissue cultures takes half a year, and generating a transgenic plant ...
This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State University. Imagine you are dealing with a defective gene, Xm, the sequence of which is identical to the correct gene, Xw, except for a single base. If you heard about CRISPR, ...
This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease of transformation. There are many motivations to generate transgenic Arabidopsis, from studying ...
This post was contributed by guest blogger Courtney Price, the Education & Outreach Specialist for the Arabidopsis Biological Resource Center and the Center for Applied Plant Sciences at The Ohio State University. Established in 1991, the Arabidopsis Biological Resource ...
Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing to its ease of use and utility in a wide variety of organisms. While much of current CRISPR research focuses on its potential ...
