Many, many techniques are available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent protein (FP) and measure the signal produced when the candidate proteins’ interaction brings the pieces of the FP ...
Selectable transgene markers for plant transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings or callus (undifferentiated plant cell culture), ...
There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of interactions between these proteins is hard to imagine yet alone study.
First described in the 1980s, protein tags are now one of the most useful items in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization of a protein of interest, purify it, or determine its expression level without the need for a ...
Protein purification can be one of the most stressful lab activities. Working with proteins requires a substantial amount of properly folded, relatively pure protein, but getting to this stage is often much easier said than done. As reviewed in our Plasmids 101 series, proteins ...
Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including (but not limited to) purification, detection, solubilization, localization, or protease protection. Thus far ...
Mark Howarth’s lab at the University of Oxford is dedicated to generating new tools to manipulate biology based on molecular features found in nature, with the ultimate goal to improve the diagnosis of disease, and cancer in particular. They recently introduced the ...
Homologous recombination is the process by which nearly all domains of life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S. cerevisiae and S. pombe. The ...
