Antibodies 101: Single Chain Fragment Variables (scFvs)

By Beth Kenkel

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Addgene mascots Blugene and Aavery holding balloons shaped like antibodies
Comparison schematic between polyclonal antibodies, monoclonal antibodies, and recombinant monoclonal antibodies
Western blot process overview
Antibody schematic. The two heavy chains form a Y shape while two shorter light chains are next to each branch of the Y shape. Antigen binding sites include the light and heavy chain at the top of the Y shape. The top part of the antibody is the Fab domain and the bottom part of the antibody is the Fc domain.
In blue light, OptoNanobodies cannot bind target protein. In darkness, OptoNanobodies bind the target.

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