This post was written by Alyssa Shepard and Angelo Nicolaci, a PhD student at Moffitt Cancer Center. Engineering usually calls to mind building things, lots of math, and maybe heavy machinery. But not all engineering is at a large scale — some is done on much smaller equipment ...
Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading controls needed for relative quantification of a western blot — and why you might want to be careful relying on so called ...
Every year, we like to add in some holiday cheer to our blog and social media. This year, we're doing so through the twelve days of antibodies. We hope you enjoy it, and we wish you a great 2025!
Thinking about using antibody-based applications in your experiment...but not sure where to start or what your options are? Addgene's latest animation is here to help!
If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options — or not know where to start looking to find one. Luckily, there are websites that can help: Antibody data repositories, which share validation data, and antibody search engines, ...
Addgene is very excited to share the very first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource for anyone looking to use antibody-based applications in their research.
Western blots can be tricky to get right. If you’ve thoughtfully done the technical design of your blot, but are still finding yourself having issues, you’re in the right place! In this post, we’ll talk about western blot optimization and troubleshooting.
If you’re using antibodies in your research, you've probably found yourself staring at a browser full of tabs, each open to a different antibody option. Or you may find yourself with only two options, but very little data on which might work in your application. It can be quite ...
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