An Introduction to Adenovirus

By Guest Blogger

Recent Posts

Viral Vectors 101: What is a Virus (and a Viral Vector)?

Rachel Leeson August 18, 2023

Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t even decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not just on the disease front.

A cartoon showing a non-enveloped virus with capsid and genome labeled (left) and an enveloped virus with capsid, genome, and envelop labeled (left.)

Open Science

Negative Can Be Positive: Open AAV Data with Addgene

Jason Nasse September 6, 2022

Working with AAV vectors in the brain and peripheral nervous system presents a special challenge to scientists. What works well in one cell or neuron type may not work well for a neighboring cell type, even within the same brain region or nucleus. Just optimizing AAV expression ...

Survey results showing scientists want more negative data in the AAV data hub

Viral Vectors

AAV Q&A with Tim Miles

Guest Blogger May 17, 2022

Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that didn’t get answered live - but he kindly took some time to address all your unanswered questions via text! (well, maybe not all of ...

CRISPR

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Kendall Morgan December 10, 2020

Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel. Adenoviral vectors (AdVs) have been used for many years for in vivo delivery and gene therapy and represent one of the most studied viruses. Adenoviruses are non-enveloped double-strand DNA viruses ...

Schematic of targeted gene knock-out (left) and targeted DNA knock-in (right) by CRISPR/Cas9. Adenoviral CRISPR vectors (left) or CRISPR vectors and donor DNA vectors (right) are transduced to target cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final step of targeted gene knock-out (left), a fragment is deleted from two homologous strands of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA knock-in, donor DNA is copied into the cut site through error-free homologous recombination (HR).

Viral Vectors

Adenoviral Vector Production and Troubleshooting

Karen Guerin June 14, 2018

Adenoviral vectors (AdV) are attractive vectors for research applications and gene therapy: they can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells, and do not integrate into the host’s genome. The main challenge with using ...