We all know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable, and it grows fast to boot. The yeast strains S. cerevisiae and S. pombe have dominated the research scene. But what about the other yeasts – in the biomedical ...
Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell, including protein-protein, protein-DNA and protein-RNA interactions. The 1980s saw a flurry of discovery in the field of ...
In our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely utilized across many different cell types and each one requires different elements for vector propagation. This post, along ...
Homologous recombination is the process by which nearly all domains of life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S. cerevisiae and S. pombe. The ...
