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K. phaffii: Rising to the Occasion

By Susanna Stroik

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Recent Posts

Yeast

Tips for Screening with Yeast Two Hybrid Systems

Jessica Welch October 22, 2015

Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen for molecular interactions in the cell, including protein-protein, protein-DNA and protein-RNA interactions. The 1980s saw a flurry of discovery in the field of ...

Yeast

Plasmids 101: Yeast Vectors

Marcy Patrick February 25, 2014

In our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely utilized across many different cell types and each one requires different elements for vector propagation. This post, along ...

Yeast

Tag Your Favorite Yeast Genes with Ease

Julian Taylor-Parker November 19, 2013

Homologous recombination is the process by which nearly all domains of life repair genomic damage, specifically double strand breaks. Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S. cerevisiae and S. pombe. The ...

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