A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

By Jennifer Tsang

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Schematic for transforming pEcgRNA and pEcCas into E. coli to edit the genome. Plasmids are subsequently cured from the cell.
Multiplexed insertion is possible using a single plasmid that contains a multi-spacer CRISPR array. This encodes separate gRNAs that target different locations in the genome.
Metagenomic Alteration of Gut microbiome by In situ conjugation addgene blog
Acoustic reporter genes graphical abstract
Microbial Warfare No Caption-01.png

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