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Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Posted by Jennifer Tsang on Sep 4, 2018 8:53:28 AM

Researchers express genes of interest from plasmids in order to study gene function or to engineer cells for specific purposes. Unfortunately, plasmid copy numbers vary within cell populations and over time resulting in variable gene expression that can impact observed phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy number in a cell.

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Topics: Plasmid Technology, Synthetic Biology, Plasmid Elements

Interview with Avital Bailen: iGEM and the OriginALS team

Posted by Tyler Ford on May 30, 2018 8:50:33 AM

In this episode of the Addgene Podcast, Addgenie Kim de Mora sits down with Avital Bailen from the "OriginALS" iGEM team at Ben-Gurion University in Beer Sheva, Israel. Avital provides a brief description of the OriginALS iGEM project below and discusses more of what she hopes to learn from the iGEM competition in the podcast.

Before we dive into the interview, we’ll briefly introduce you to Kim and iGem. By the end of this interview, we hope you’ll have a good understanding of why iGEM is an important component of scientific training for many researchers and of how the goals of iGEM and Addgene intersect in concretely useful ways for iGEM participants.

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Topics: Education, Synthetic Biology, Podcast

Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis

Posted by Tyler Ford on Nov 29, 2017 9:00:00 AM

Today’s episode of the Addgene Podcast features guest host and Addgene European Outreach Scientist, Benoit Giquel. Traveling from our offices just outside of London, it was a short journey for Benoit to interview Addgene Advisory Board Member and lead researcher at Imperial College London, Dr. Tom Ellis. Dr. Ellis does research in synthetic biology and bioengineering including a recent effort to construct a synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes, and obstacles to becoming an academic researcher.

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Topics: Synthetic Biology, Podcast

A Deep Dive into BioBuilder

Posted by Guest Blogger on Oct 11, 2017 9:44:02 AM

This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of synthetic biology into the hands of students and teachers in the U.S. and beyond.

“I want to learn more about Synthetic Biology. Can you tell me where to start?” is a question more and more students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I’m trying to inspire them and also keep up with all the new biotechnology.”

These are the types of inquiries that routinely hit the inbox at the BioBuilder Educational Foundation. With a goal of “bringing tomorrow’s science into today’s classrooms,” BioBuilder answers the call by offering a variety of educational programs all focused on current questions and experiments in the field of synthetic biology.

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Topics: Science Communication, Synthetic Biology, Education

Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

Posted by Tyler Ford on Dec 20, 2016 10:30:00 AM

In this episode of the Addgene Podcast, we sit down with Kwabena Duedu, a researcher at the University of Health and Allied Sciences in Ghana. He’s done research at a number of institutions in Ghana and most recently got his PhD in cell and molecular biology at the University of Edinburgh where he worked on developing novel systems for bioconversion of cellulosic biomass to useful products under Professor Christopher E. French.


Dr. Duedu was recently awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For this grant, we’re working with fellow nonprofit, Seeding Labs, to distribute plasmids to researchers in developing regions and thereby accelerate their research. Kwabena came across the plasmid grant in Seeding Lab’s Newsletter and recently visited Cambridge to spend some time learning and doing research at Novartis.

Listen to learn how Kwabena plans to use his research experiences as well as plasmids from Addgene to bring new opportunities to Ghanaian scientists

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Topics: Podcast, Interview, Synthetic Biology

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