Plasmids 101: Modular Cloning Applications and Kits

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Graphic showing the workflow for high complexity Golden Gate Assembly, starting with destination vector and PCR amplification of fragments, which combined in a single-tube reaction with enzyme and DNA ligase to produce the assembled DNA product.
Copy of 2016_4_28_PlasmidClock_TJF-01
Flowchart for choosing a cloning method

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