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It’s CRISPR Week at Addgene!

Posted by Jennifer Tsang on May 13, 2019 8:01:45 AM

In 2012, we received the first CRISPR plasmid deposit. Now we have over 8,000 CRISPR plasmids in the repository that have been shared over 140,000 times to over 75 countries. That’s a lot of CRISPR! As we’ve shared these tools over the years, the amount of CRISPR educational resources hosted on our website and blog have grown. We even have a CRISPR eBook. This week, we’ll continue to grow the ways we share science by hosting our first ever CRISPR Week on social media.

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Topics: CRISPR

Visualizing Genomic Loci with CRISPR-Sirius

Posted by Jennifer Tsang on Apr 18, 2019 8:53:21 AM

The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has been widely adapted for CRISPR activation and inhibition experiments. But over the past few years, dCas9 has become a robust visualization tool to study the spatial and temporal arrangement of chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools is CRISPR-Sirius, the brightest CRISPR-based tool to date for visualizing genomic loci in living cells.

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Topics: CRISPR

Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

Posted by Beth Kenkel on Apr 4, 2019 8:53:46 AM

The vast majority of bacteria are undomesticated which limits the tools scientists can use to study them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain, such as a B. subtilis, or a narrow range of bacterial species, such as the human gut bacteria B. thetaiotaomicron, Mycobacterium, Pseudomonas, and E. coli. However, many non-model bacterial species lack genetic tools.

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Topics: CRISPR

What's New in CRISPR - Spring 2019

Posted by Jennifer Tsang on Mar 21, 2019 8:47:48 AM

In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research.

 

 

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Topics: CRISPR

Targeted Mutagenesis with EvolvR

Posted by Beth Kenkel on Feb 21, 2019 8:15:26 AM

Mutagenesis is a tool that both evolution and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify and/or facilitate the evolution of new phenotypes, and forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries exist, but none provide a means to continuously diversify all nucleotides within a user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber Lab at Berkeley, provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications.

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Topics: CRISPR

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