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The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

Posted by Guest Blogger on Jan 21, 2020 9:30:00 AM

This post was contributed by the Open Repository of CRISPR Screens.

Imagine you’ve just discovered that your favorite gene was described in a CRISPR screen publication. You see this mentioned in the results section, but you had to dig through the supplemental files to see the actual data. Now you’re wondering if your favorite gene is included in other CRISPR screens and whether that data is useful for your research. Fortunately, there’s a growing open repository of CRISPR screen data that might be able to help you out.

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Topics: CRISPR, Other CRISPR Tools

What's New in CRISPR - December 2019

Posted by Jennifer Tsang on Dec 17, 2019 9:37:19 AM

In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth (such as these recent blog posts on prime editing, IgnaviCas9, and Nanoblades), but we hope that this blog series will help you find more new CRISPR tools for your research!

This time:

  • CRISPRi in Burkholderia
  • Base editors
  • CRISPR toolkit for epitope tagging
  • Lentiviral capsid-based Cas9/gRNA ribonucleoprotein delivery
  • Cytosine deaminase for RNA editing
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Topics: CRISPR, Other CRISPR Tools

Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

Posted by Christina Mork on Nov 12, 2019 9:00:00 AM

In 2008 the Quake Lab at Stanford University became interested in exploring biological dark matter – large tracts of the microbial tree of life that remained unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA sequencing, hot springs were known to be diversity hotspots containing abundant biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (YNP).

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Topics: CRISPR, Cas Proteins

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Posted by Andrew Hempstead on Nov 5, 2019 8:28:59 AM

In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment? The three years flew by, but since then, scientists have adapted CRISPR nucleases for many more specific research needs. In this update, we will focus on the most recent advances and how some of these variants may be appropriate for your specific research question.

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Topics: CRISPR, Cas Proteins

Prime Editing: Adding Precision and Flexibility to CRISPR Editing

Posted by Jennifer Tsang on Oct 24, 2019 9:26:53 AM

There are over 75,000 pathogenic genetic variants that have been identified in humans and catalogued in the ClinVar database. Previously developed genome editing methods using nucleases and base editors have the potential to correct only a minority of those variants in most cell types. A new technique from David Liu’s lab at the Broad Institute could add more precision and flexibility to the CRISPR editing world.

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Topics: CRISPR, Cas Proteins, CRISPR gRNAs, Base Editing

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