If you’re looking for an antibody that works for your experiment, you may be overwhelmed by options — or not know where to start looking to find one. Luckily, there are websites that can help: Antibody data repositories, which share validation data, and antibody search engines, which allow you to search many different antibody catalogs at the same time.
If you're looking for an antibody, especially a validated antibody, we recommend using antibody search engines and antibody data repositories to find an antibody for your experiment. Search engines let you easily find and compare available antibodies from many vendors, while data repositories share validation and experimental data to help you decide if the antibody is a good fit for your experiment. They come in all sizes and specificities, from target-specific ones to application-specific ones to anything goes. We've shared the ones we're familiar with in Table 1.
Table 1: Antibody data repositories and search engines
Website |
Targets |
Application |
Purpose |
Notes |
Any |
Any |
Data repository |
||
Human proteins |
Immunoblot (western blot), IP, and IF. |
Data repository |
||
Healthy human cells |
Imaging (IHC, ICC, IF, etc.) |
Data repository |
||
Cancer |
Any |
Data repository |
||
Immune cells |
Flow cytometry (potentially others) |
Data repository |
||
Any (see notes) |
Any |
Data repository |
Only accepts data for antibodies in Addgene’s repository |
|
Any |
IBEX multiplex tissue imaging |
Data repository |
||
Mouse and human brain |
IHC |
Data repository |
||
Any |
Any |
Search engine |
May include validation data |
|
Any |
Any |
Search engine |
May include validation data |
|
Any |
Any |
Search engine |
Are we missing an antibody data repository or search engine that you use? Let us know in the comments so we can add it to the table! And if you're interested in joining discussions about improving antibody quality throughout research, consider checking out the Only Good Antibodies community on LinkedIn.
Topics: Antibodies, antibodies 101
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