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Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’s so important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its ...
This post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik. The advent of CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified to enable researchers to knock out, knock in, base ...
This post was contributed by guest blogger Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows ...
Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is unique compared to the rest of the genome and (2) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM ...
Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for ...
This post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-CoV-2 has spread to almost every part of the world manifesting as a full-fledged pandemic. Containing the spread of this virus has become an utmost priority for countries around ...
Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection is crucial for clinical diagnostics, genotyping, and biotechnological advancements. Many methods of nucleic acid detection however, either lack the sensitivity or the ...
