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Overcoming the Challenges of Lentiviral Production

Posted by Meghan Rego on Aug 7, 2018 8:31:12 AM

While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges. Whether choosing a system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning selection and expression in your target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide an overview of some of the common challenges associated with producing and using lentivirus and offer some tips and tricks for overcoming these hurdles.

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Topics: Viral Vectors, Viral Vectors 101, Viral Vector Protocols and Tips, Retroviral and Lentiviral Vectors

Popular Retroviral Vectors and Their Uses in Scientific Research

Posted by Susanna Bachle on Jul 24, 2018 8:51:21 AM

Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make excellent tools for genetic engineering. There are several different viral vectors to choose from, for example gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments best have a look at this viral vector overview.

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Topics: Viral Vectors, Retroviral and Lentiviral Vectors

Quick Guide to All Things Lentivirus

Posted by Benoit Giquel on Mar 21, 2017 10:30:00 AM

If you are interested in using lentiviral vectors to introduce your favourite gene into your favourite cell line or into primary cells, this blog article will give you some tips to plan your experiment using the lentiviral vector system.

Viral vectors have been increasingly popular in fundamental and applied research since their first use in the early 90’s to genetically modify primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful due to their ability to infect both dividing and non-dividing cells, including stem cells. These properties make lentiviral vectors fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors.

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Topics: Viral Vectors, Retroviral and Lentiviral Vectors

Lentiviral Vector Uses and Overview

Posted by Mary Gearing on May 19, 2016 10:30:00 AM


Lentiviral vectors
are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications, and they’ve also had success in clinical gene therapy applications. Read on to learn more about the current (and future) applications of lentiviral vectors!

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Topics: Viral Vectors, Viral Vectors 101, Retroviral and Lentiviral Vectors

Getting the Most from Your Lentiviral Transduction

Posted by Meghan Rego on Apr 7, 2016 10:30:00 AM

Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest. While the general approach for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting the most out of your lentiviral transduction experiments.

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Topics: Viral Vectors, Viral Vector Protocols and Tips, Retroviral and Lentiviral Vectors

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