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The widespread use of γ-retroviruses (gamma-retroviruses) in cancer and stem cell research has prompted the development of multiple virus packaging methods. Across these methods, the following components are needed:
Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t even decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not just on the disease front.
The Retroviridae (commonly called retrovirus) family — of which HIV is a member — may seem like an unlikely candidate to use as a viral vector, but retroviruses have been developed into some of the most widely used tools in molecular biology. “Retroviruses” is an umbrella term ...
If you’ve ordered a pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through ...
To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor pairings are broad, allowing delivery into many cell types, while others are specific, allowing delivery ...
While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges. Whether choosing a system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning selection and expression in your target cell ...
Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make excellent tools for genetic engineering. There are several different viral vectors to choose from, for example gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated ...
