New Viral Vectors - Winter 2025

Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available in the Addgene viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage and a blanket, then scroll on for some wintery delights! 

Table 1: New ready-to-use viral vectors in the Addgene repository 

Name	Serotype	Category	Depositor	Notes
pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag	AAV5	Biosensor	Tim Brown	New viral prep
pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE	AAV1, AAV5	Biosensor	GENIE Project	New viral prep with multiple serotypes
pAAV-hSyn-GRAB_OTmut	AAV1	Biosensor	Yulong Li	New viral prep
pAAV-CAG-NIR-GECO2G	AAV1, AAV2, AAV9	Biosensor	Robert Campbell	New viral prep with multiple serotypes
AAV-hSyn-Ribo-jGCaMP8s	AAV1	Biosensor	Marianne Fyhn	New viral prep
AAV-hSyn-Ribo-jGCaMP8m	AAV1	Biosensor	Marianne Fyhn	New viral prep
pAAV-Ef1a-fDIO EYFP	AAV5. AAV8, AAVrg	Control	Karl Deisseroth	New serotype (multiple)
rAAV-CAG::FLEX-rev:: hM4D-2a-GFP	AAV1	Chemogenetics	Scott Sternson	New viral prep
pAAV-CaMKIIa-hM3D(Gq)-mCherry	AAV1	Chemogenetics	Bryan Roth	New serotype
pAAV-CaMKIIa-hM4D(Gi)-mCherry	AAV1	Chemogenetics	Bryan Roth	New serotype
pAAV-hSyn-hM3D(Gq)-mCherry	AAV1	Chemogenetics	Bryan Roth	New serotype
pAAV-hSyn-hM4D(Gi)-mCherry	AAV1	Chemogenetics	Bryan Roth	New serotype
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato	AAV8	Molecular tool	Massimo Scanziani	New viral prep
pAAV_hSyn1-SIO-stGtACR2-FusionRed	AAV5	Optogenetics	Ofer Yizhar	New serotype
pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE	AAV8	Optogenetics	Karl Deisseroth	New viral prep
pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE	AAVrg	Optogenetics	Ofer Yizhar	New serotype
pAAV-syn-Flpo	AAVrg	Recombinase	Ian Wickersham	New viral prep
pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40	AAV2	Recombinase	James M. Wilson	New serotype
pENN.AAV.hSyn.Cre.WPRE.hGH	AAV2	Recombinase	James M. Wilson	New serotype
pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40	AAVrg	Recombinase	James M. Wilson	New serotype
pENN.AAV.CamKII 0.4.Cre.SV40	AAVrg	Recombinase	James M. Wilson	New serotype
AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237)	AAV-PHPeB	Control	Jonathan Ting	New viral prep
AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787)	AAV-PHPeB	Control	Jonathan Ting	New viral prep
AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610)	AAV-PHPeB	Control	Jonathan Ting	New viral prep
AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755)	AAV-PHPeB	Optogenetics	Jonathan Ting	New viral prep
AiP14033: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033)	AAV-PHPeB	Optogenetics	Jonathan Ting	New viral prep
AiP14035: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035)	AAV-PHPeB	Optogenetics	Jonathan Ting	New viral prep
AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496)	AAV-PHPeB	Control	Jonathan Ting	New viral prep
AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825)	AAV-PHPeB	Recombinase	Jonathan Ting	New viral prep
AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140)	AAV-PHPeB	Optogenetics	Jonathan Ting	New viral prep
pAAV_BiSSTe10_dTomato_nlsdTomato	AAV-PHPeB	Control	Gordon Fishell	New viral prep
pAAV_BiSSTe10_ChR2_mCherry	AAV-PHPeB	Optogenetics	Gordon Fishell	New viral prep
pAAV_BiCHATe27_dTomato_nlsdTomato	AAV-PHPeB	Control	Gordon Fishell	New viral prep
pAAV_BiCHATe27_ChR2_mCherry	AAV-PHPeB	Optogenetics	Gordon Fishell	New viral prep
pAAV_BiVIPe4_dTomato_nlsdTomato	AAV-PHPeB	Control	Gordon Fishell	New viral prep
pAAV_BiVIPe4_ChR2_mCherry	AAV-PHPeB	Optogenetics	Gordon Fishell	New viral prep
pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato	AAV-PHPeB	Control	Gordon Fishell	New viral prep
pAAV_BiLAMP5e3_ChR2_mCherry	AAV-PHPeB	Optogenetics	Gordon Fishell	New viral prep
pAAV_BiPVe4_dTomato_nlsdTomato	AAV-PHPeB	Control	Gordon Fishell	New viral prep
pAAV_BiPVe4_ChR2_mCherry	AAV-PHPeB	Optogenetics	Gordon Fishell	New viral prep
pAAV_BiPVe3_dTomato_nlsdTomato	AAV-PHPeB	Control	Gordon Fishell	New viral prep
pAAV_BiPVe3_ChR2_mCherry	AAV-PHPeB	Optogenetics	Gordon Fishell	New viral prep
pAAV_BiSSTe4_dTomato_nlsdTomato	AAV-PHPeB	Control	Gordon Fishell	New viral prep
pAAV_BiSSTe4_ChR2_mCherry	AAV-PHPeB	Optogenetics	Gordon Fishell	New viral prep

AAVrg = AAV retrograde

Looking for more viral vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers over 3,000 eligible transfer plasmids and five different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted viral vector reports. And don't forget to use the link below to keep up with the latest in Addgene's viral vector services and resources!  

This blog post was written by Rachel Leeson and Brian O'Neill. 

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