By Emily P. Bentley
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I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020. As I left the ivory tower and walked down into its foothills, I began to have conversations with many people who eagerly follow progress in synthetic biology, and want to be ...
Welcome to our deep dive series, which aims to increase your understanding and technical proficiency with common applications - now let’s dive right in!
In a lab, you may have heard the phrase gram negative or positive being used to describe a species of bacteria, but what does it actually mean? What relevance does it have on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the ins ...
Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using antibodies to capture protein:DNA complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ...
New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the Lab Bench series was born from the shift to remote learning due to COVID-19. We had many protocols for plasmid cloning and working with viral vectors, but what we lacked were ...
What is polymerase chain reaction? If you have ever worked in a molecular biology laboratory you have likely done a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied many many times in a short time period. PCR was invented in ...
You’ve gotten the plasmid encoding your protein of interest from Addgene, transfected it into your target cells, now what? How can you tell if the protein you are so keen to study is expressing in your cells? Immunoblotting or simply the western blot, or western, is one of the ...
