By Alyssa Shepard
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Previously, we provided a general overview of the QC process at Addgene. All plasmids go through this same initial QC process using an Illumina MiSeq, but that’s not the only technology Addgene uses to ensure accuracy of deposited plasmids. To resolve QC issues, assembly issues, ...
Nobody’s perfect! This is a platitude I’m sure we have all heard. It’s a truth that extends to both people and the tools we use. Scientists utilize an array of tools that rely on DNA amplification and manipulation to produce and edit plasmids. These are prone to error — both ...
Since its early years, the light microscope has been a powerful tool in almost all disciplines. In biology, innovative new imaging approaches are constantly being developed which combine computational processing with biological protocols and revolutionary physical approaches. ...
Working with AAV vectors in the brain and peripheral nervous system presents a special challenge to scientists. What works well in one cell or neuron type may not work well for a neighboring cell type, even within the same brain region or nucleus. Just optimizing AAV expression ...
As a scientist, perhaps the most important part of your research is communicating the results of the work. Yes, those control experiments are important — but without letting other scientists know the results, your work won’t have any impact!
This post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. From the perspective of an author submitting a paper, the peer-review seems like another dragon to slay on the way to publish your work in a scientific journal. The peer-review is ...
The C. elegans community has always emphasized the need for open science and collaboration. The field already has comprehensive reference pages and curated databases for scientists including Wormbook, Wormatlas and Wormbase. And scientists have been continuously sharing their ...
