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Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading controls needed for relative quantification of a western blot — and why you might want to be careful relying on so called ...
We recently updated our blog post on Prime Editing, and that meant rereading many of the original papers reporting various prime editing tools. These papers are chock full of great tips to guide your experimental design, especially the design of the RNA sequences you’ll use in ...
I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020. As I left the ivory tower and walked down into its foothills, I began to have conversations with many people who eagerly follow progress in synthetic biology, and want to be ...
Welcome to our deep dive series, which aims to increase your understanding and technical proficiency with common applications - now let’s dive right in!
In a lab, you may have heard the phrase gram negative or positive being used to describe a species of bacteria, but what does it actually mean? What relevance does it have on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the ins ...
Chromatin immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using antibodies to capture protein:DNA complexes with antibodies specific to your protein(s) of interest. Once the complex is captured, it is ...
New to the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the Lab Bench series was born from the shift to remote learning due to COVID-19. We had many protocols for plasmid cloning and working with viral vectors, but what we lacked were ...
