This post was written by Angelo Nicolaci, a PhD student at Moffitt Cancer Center.
You have just purified your plasmid and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA you have or how pure your sample really is?
CoPurifying plasmid DNA from bacterial cultures is a core protocol in molecular biology. And whilekit-free plasmid purification is always an option, let’s be honest — kits are quick, easy, and don’t involve phenol-chloroform.
Ah, the notorious western blot: we meet again. So useful, yet so finicky to design and optimize. Today we’ll cover the normalization and loading controls needed for relative quantification of a western blot — and why you might want to be careful relying on so called ...
We recently updated our blog post on Prime Editing, and that meant rereading many of the original papers reporting various prime editing tools. These papers are chock full of great tips to guide your experimental design, especially the design of the RNA sequences you’ll use in ...
If you’re looking for the best way to improve your lab techniques — but perhaps are a little limited on time — check out Addgene’s new Quick Tip series on YouTube!
Western blots are a great tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a lot of work — and what if you want to detect more than one protein in your sample? Or what if something weird happened during your western and your ...
Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."
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