By Emily P. Bentley
Read More
Over 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database. Previously developed genome editing methods using nucleases and base editors have the potential to correct only a minority of those variants in most cell types. But ...
Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019; Chen & Liu, 2023). This method uses a prime editor (typically ...
The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg lab (also called the ...
Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan Ward who is an assistant professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments possible in ...
This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA transposons are ubiquitous genetic elements capable of spreading within and between genomes, and have been adapted for a wide variety of ...
This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020. Scientists around the world have been making major improvements to CRISPR technology since its initial applications for ...
Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation, but enhancers and chromatin looping play important supporting roles. Enhancers are cis regulatory DNA sequences that, when bound by transcription factors, can increase a ...
