Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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Graphic of channelrhodopsin activated with blue light. Without light, the channelrhodopsin is inactive, no ions flow in, and neurons don't fire. With blue light, the channelrhodopsin opens and allows an influx of ions into the neuron. This results in neuronal firing.
In blue light, OptoNanobodies cannot bind target protein. In darkness, OptoNanobodies bind the target.
FLEx-switch
Optogenetics-Zebrafish-Behavior

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