By Beth Kenkel
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Originally published Mar 8, 2016 and last updated Sept 3, 2020 by Nyla Naim. Scientists around the world have been making major improvements to CRISPR technology since its initial applications for genome engineering in 2012. Many of these advances have stemmed from the goal of ...
Chemogentic and optogenetic technologies have pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While they serve similar purposes, both techniques offer researchers different advantages and limitations. The four main factors in which ...
It’s time to choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have two options: Option #1: Affinity tag purification You tag YFP and use an affinity column for purification. After binding YFP to the column, you wash several ...
This post was contributed by Alessia Armezzani, scientific communication manager at genOway. A few decades ago, the brain remained elusive, not from a lack of intellectual curiosity on the part of scientists but, rather, from the limited technologies available. Over the past few ...
Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers to silence or incite neuronal firing and study subsequent effects on behavior. The system is an especially powerful tool for in vivo behavioral studies because it is ...
This post was contributed by guest blogger Derek Simon. The actual experiments you do will be determined by the topic you’re interested in studying, but, in today’s post, we’ll discuss some of the important considerations you should think about when developing optogenetics ...
This post was contributed by guest blogger Derek Simon. The surgeries and standard molecular neuroscience validation experiments we discussed last week are only half of the battle when using optogentics to answer a research question. The flip side of the optogenetics coin is ...
