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Over 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database. Previously developed genome editing methods using nucleases and base editors have the potential to correct only a minority of those variants in most cell types. But ...
You’ve probably heard that only 2% of our genome is made of protein-coding genes, and you might be wondering what the rest of our genome could possibly be made up of. The answer is… drum roll please… non-coding RNAs! You probably didn’t see that coming, right? Non-coding RNAs ...
CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing tool… but ...
Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’s so important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its ...
The PAM… that sneaky little bit of sequence that you hope is present next to the "perfect" guide sequence for your genome engineering experiment. With CRISPR entering the clinic for correction of disease-causing alleles, and the growing need for gene editing in research, the old ...
“Off-target effects” a vague catch-all term for unintended consequences in an experiment. For CRISPR work, this often means DNA cleavage at unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand them in order to design the ...
¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español!
