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Taking the road less traveled and generating a knock-in cell line instead of a knock-out? We’ve got you covered in this blog, with tips and tricks for harnessing the homology-directed repair pathway, designing the best donor DNA, and avoiding common mishaps in this class of ...
We are all oh so familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas family of proteins has many useful genome engineering enzymes, each with their own unique features and quirks. Here, we will review the genome engineering ...
This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA and introduced it into your target cells with Cas9. Hooray! Now it’s time to make sure your genome edits went according to plan. In this blog post we’ll explain ...
This post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik. The advent of CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified to enable researchers to knock out, knock in, base ...
Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp new CRISPR material for you! The CRISPR field moves fast. That’s why we’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook ...
Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple ...
Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for ...
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