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To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor pairings are broad, allowing delivery into many cell types, while others are specific, allowing delivery ...
Your next cool experiment requires some AAV. Where do you start? Plasmids of course! You just need three plasmids to start making the AAVs you need for your experiment: the packaging plasmid which contains the AAV structural and packaging genes, the adenoviral helper plasmid ...
So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with AAV before, but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making AAV starts with some plasmids. You just need three ...
In order to understand the complex wiring of the brain and the underpinnings of neurological disease, neuroscientists need to be able to probe cells and circuits without disrupting normal brain function. Chemogenetics has become a popular tool in the field as it provides a ...
My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, I had never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great co-workers ...
While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges. Whether choosing a system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning selection and expression in your target cell ...
Many of us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells, titer, the amount of virus used, really does matter. (*psst*, quality definitely also ...
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