Viral Vectors 101: AAV Serotypes and Tissue Tropism

By Emily P. Bentley

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Schematic depicting HEK-293T cells in a culture dish and Sf9 cells in a culture flask being used to produce various viruses
A line and box diagram representing the genome, with relevant genes represented by labeled boxes, of gamma-retrovirus with five gene represented by boxes,
A flow chart showing the steps of rAAV production. Details in blog post.
 A viral particle enters a host cell, enters the host nucleus, is transcribed into RNA, and is translated into protein.
Cartoon schematic: a barcoded transfer plasmid and packaging plasmids are put in barcoded LentiX-293T cells. From this, an arrow points to virus particles with a barcode; an arrow from the barcode points to

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