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Tips for a 1st time AAV user (by a Rookie AAV user)

Posted by Beth Kenkel on Oct 23, 2018 7:49:25 AM

My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, I had never worked with AAV, so naturally I had to package some virus for my first experiment. It was a bit intimidating, but I had my lab’s protocols and some great co-workers to help me out. Even with these tools, I found myself writing AAV production tricks into the margins of my protocol. While these tips weren’t critical to the experiment, they definitely made my life easier!  In this post, I’ll share some AAV production, purification, and titration tips, while also summarizing the basic steps and analyses needed for packaging AAV.

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Topics: Viral Vectors, Viral Vectors 101, Viral Vector Protocols and Tips, AAV

Overcoming the Challenges of Lentiviral Production

Posted by Meghan Rego on Aug 7, 2018 8:31:12 AM

While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges. Whether choosing a system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning selection and expression in your target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide an overview of some of the common challenges associated with producing and using lentivirus and offer some tips and tricks for overcoming these hurdles.

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Topics: Viral Vectors, Viral Vectors 101, Viral Vector Protocols and Tips, Retroviral and Lentiviral Vectors

The Importance of High Titer for AAV Transductions

Posted by Luke Hanley on Mar 1, 2018 9:11:54 AM

Many of us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns out this isn’t the case for using AAV. When it comes to infecting cells, titer, the amount of virus used, really does matter. (*psst*, quality definitely also matters).

Check out this post for a refresher on AAV titers

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Topics: Viral Vectors, Viral Vectors 101, Viral Vector Protocols and Tips, AAV

AAV Titers: Where do they come from and what do they mean?

Posted by Leila Haery on Nov 15, 2017 10:00:00 AM

Remember the game show “The $25,000 Pyramid” where one player tries to get the other to guess a category by listing off things that fall into that category? Okay, let’s play. I’ll list the examples and you try to guess the category:

ELISA...
qPCR...
Digital droplet PCR...
DNA dot blot...
Transduction assay...
SDS-PAGE...
Electron microscopy…

Any guesses?

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Topics: Viral Vectors, Viral Vectors 101, AAV

Beginner's Guide to Viral Vectors

Posted by Leila Haery on Jun 2, 2017 10:30:00 AM

You can use viral vectors for many experimental purposes. To help you make sense of all the viral vector information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide. Print out the guide and use it for quick reference when you're designing your next virus experiment.

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Topics: Viral Vectors, Viral Vectors 101

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