By Baiyang Liu and James Chappell, Rice University. For decades, we’ve been designing experiments around two major limitations of plasmids: copy number and incompatibility. While functional, such workarounds are clunky. To address this, we created a synthetic origin of ...
Who doesn’t love a library? A traditional library might contain thousands or millions of different books. Addgene’s pooled libraries are a little different: each is a collection of plasmids that share the same backbone and differ only in a small region. This makes them useful ...
You have just purified your plasmid and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA you have or how pure your sample really is?
An oldie but a goodie — a phrase used to refer to something that has fallen out of fashion but is still useful and most importantly, effective. In the research world, there is a myriad of tools this could be applied to. When it comes to altering gene expression, CRISPR ...
CoPurifying plasmid DNA from bacterial cultures is a core protocol in molecular biology. And whilekit-free plasmid purification is always an option, let’s be honest — kits are quick, easy, and don’t involve phenol-chloroform.
If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis virus, you may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control exceptions were made by the US Bureau of Industry ...
Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy in research, but we’re really borrowing these tools from Mother Nature’s toolbox. Natural plasmids, also called environmental plasmids, are all around us!
If cloning methods had personalities, SLIC (sequence- and ligation-independent cloning) would be a true rebel. Not only does this system not use site-specific recombination, it also doesn’t require a ligation step. Based on the robust system of homologous recombination found in ...
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