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Turn it on? Turn it off? Turn it down, but not forever? You don’t need to resort to the force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this post, we will review tried-and-true protein expression control tools as well as ...
What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used to study gene expression in cells. But these reporters have limited use in vivo because they are based on light which is very easily scattered by tissue. Ultrasound, on the ...
This post was originally written by Mary Gearing, in 2018, and updated by Hannah Dotson in Dec. 2022. Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription of a target gene, it therefore determines the timing ...
In a lab, you may have heard the phrase gram negative or positive being used to describe a species of bacteria, but what does it actually mean? What relevance does it have on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the ins ...
FLIS and Flies Drosophila, more commonly known as fruit flies, have been an important animal model for research, particularly genetics research, for decades. Its short life cycle, reasonably close genetic homology to humans, and small size have allowed scientists to use it as a ...
Selectable transgene markers for plant transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection using resistance genes usually involves killing the untransformed seedlings or callus (undifferentiated plant cell culture), ...
Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need.
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