Plasmids 101: Broad Host Range Plasmids

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Schematic showing the three assembly levels of MoClo. Level 1 has 7 individual parts on a plasmid; Level 1 has seven parts all together on on plasmid; Level 2+ has three sets of five parts each on a plasmid, with two additional parts on the plasmid but separated from the sets.
Cover page of the plasmids 101 eBook
Graphic showing the workflow for high complexity Golden Gate Assembly, starting with destination vector and PCR amplification of fragments, which combined in a single-tube reaction with enzyme and DNA ligase to produce the assembled DNA product.
Cartoon showing how GCE4All works
a screenshot of data displayed on the Addgene Request dashboard
MORF library workflow

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