Latest Posts

All Posts

DNA Purification Without a Kit

Posted by Beth Kenkel on Feb 11, 2020 9:15:00 AM

Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit. DNA purification kits have advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due to their expense and requirement for lab equipment. Plus they create plastic waste. Kits can also have the annoying tendency to runout right when you need them and to accumulate a bunch of unused buffers because you’ve run out of columns.

Read More >

Topics: Plasmid Protocols and Tips, Plasmids

Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

Posted by Various Addgenies on Dec 10, 2019 8:58:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

Read More >

Topics: Other Plasmid Tools, Plasmids

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Posted by Alyssa Cecchetelli on Dec 2, 2019 9:00:16 AM

There are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, 2013). The number of interactions between these proteins is hard to imagine yet alone study.

Read More >

Topics: Plasmid Tags, Plasmids

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Posted by Beth Kenkel on Nov 19, 2019 9:08:50 AM

It’s time to choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have two options: 

Option #1: Affinity tag purification

You tag YFP and use an affinity column for purification. After binding YFP to the column, you wash several times to remove non-specific proteins, and then elute YFP. 

Option #2: Opto-Nanobodies (OptoNBs) purification

You skip adding a tag to YFP and instead use OptoNBs. You fill a column with OptoNB coated beads and wrap the column with blue LED lights. When you switch off the lights, OptoNBs bind YFP and non-specific proteins flow through. To elute YFP, you turn on the blue lights.

Which option do you choose? 

Read More >

Topics: Optogenetics, Other Plasmid Tools, Plasmids

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Posted by Guest Blogger on Oct 17, 2019 8:28:58 AM

This post was contributed by Alessia Armezzani, scientific communication manager at genOway.

A few decades ago, the brain remained elusive, not from a lack of intellectual curiosity on the part of scientists but, rather, from the limited technologies available. Over the past few years, however, remarkable technological advances have taken researchers to the threshold of a revolution in modern neuroscience, an era in which technologies like FLEx and optogenetics meet, allowing scientists to investigate the fundamental basis of both brain function and dysfunction.

First, let’s start with the basics: What is optogenetics?

Read More >

Topics: Optogenetics, Other Plasmid Tools, Plasmids

Click here to subscribe to the Addgene Blog
 
Subscribe

 

All Topics

see all

Recent Posts