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RNA Extraction Without A Kit

Posted by Leah Schwiesow on Mar 24, 2020 9:15:00 AM

As with DNA isolation, scientists commonly rely on RNA isolation kits to make their life easier. Recently, we published a blog on DNA purification without a kit that outlined several reasons why doing something without a kit has advantages: less plastic waste, less expense, and less of being left with a bunch of random solutions when all the spin columns run out. In this article, we cover the basics of isolating RNA without a kit.

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Topics: Molecular Biology Protocols and Tips, Plasmids

Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Posted by Various Addgenies on Mar 3, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

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Topics: Hot Plasmids, Other Plasmid Tools, Plasmids

A History of Genome Engineering in Popular Culture

Posted by Guest Blogger on Feb 25, 2020 9:15:00 AM

This post was contributed by Kartik Lakshmi Rallapalli, a graduate student at the University of California, San Diego.

The revolution in genetic engineering techniques is a speculation of yesteryear which has been realized recently. Science Fiction (SciFi) writers have been curious about the capability of transforming the genetic code of living organisms and its societal implications even before the discovery of genes themselves. 

How has scientific progress in genetic editing impacted the world of fiction and vice versa? We draw a parallel between the timelines of the scientific advancements and the noteworthy work of fiction that it inspired. CAUTION: Spoilers alert!

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Topics: Genome Editing, Plasmids

DNA Purification Without a Kit

Posted by Beth Kenkel on Feb 11, 2020 9:15:00 AM

Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit. DNA purification kits have advantages: they are convenient and provide uniform, consistent results. But they are also less accessible due to their expense and requirement for lab equipment. Plus they create plastic waste. Kits can also have the annoying tendency to runout right when you need them and to accumulate a bunch of unused buffers because you’ve run out of columns.

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Topics: Molecular Biology Protocols and Tips, Plasmids

Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

Posted by Various Addgenies on Dec 10, 2019 8:58:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

Read More >

Topics: Other Plasmid Tools, Plasmids

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