Latest Posts

All Posts

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Posted by Angela Abitua on Jun 18, 2020 9:15:00 AM

Nanobodies from camelids have gotten a lot of press for being a promising approach for treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the virus to enter host cells. To do this, they injected the spike protein from two different coronaviruses into a llama over several days and isolated special camelid antibodies that bind to the spike proteins in cell culture, and inhibit these viruses. 

Read More >

Topics: Other Plasmid Tools, Plasmids, COVID-19

Plasmid Preps: Different Purity, Different Quantities, Different Uses

Posted by Paolo Colombi on Jun 16, 2020 9:25:46 AM

Not all plasmid preps are the same. Before purifying a plasmid from a bacterial culture, it is important to consider your experiment.  It will dictate the amount of DNA you need, and at which level of purity. Based on these premises we can classify a plasmid preparation in 3 different ways:

  • Transformation grade DNA
  • Cloning grade DNA
  • Transfection grade DNA
Read More >

Topics: Plasmid Cloning, Plasmids

What's the Best Way to Elute and Store Your Plasmid DNA?

Posted by Paolo Colombi on Jun 4, 2020 9:22:22 AM

If you use a kit for DNA purification or if you use a DIY purification protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols that recommend eluting in water, Tris-EDTA (TE), just Tris buffer, or some other variations. Does it make a difference? Short answer: yes. This last step will influence the stability of your sample over time and determine which experiments you can effectively use the DNA.

Read More >

Topics: Molecular Biology Protocols and Tips, Plasmids

Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Posted by Various Addgenies on Jun 2, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

Read More >

Topics: Hot Plasmids, Other Plasmid Tools, Plasmids

Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Posted by Various Addgenies on May 19, 2020 9:15:00 AM

By Will Arnold and Shreya Vedantam

Accelerating research by improving the availability of materials is a core part of our mission. In no situation is this more true than the current global pandemic in which scientists race to find vaccines, therapeutics, and other treatments to stem the spread of COVID-19. That’s why we’re proud to be working with Ginkgo Bioworks to synthesize and share SARS-CoV-2 plasmids to help academic and industrial labs accelerate research and to help find a faster solution to the COVID-19 pandemic. By the time the project is complete, Ginkgo will have synthesized approximately 3,000 constructs to support SARS-CoV-2 research.

Read More >

Topics: Plasmids, COVID-19

Click here to subscribe to the Addgene Blog
 
Subscribe

 

All Topics

see all

Recent Posts