Newly Updated AAV Data Hub!

We're excited to roll out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share their AAV validation data. The Data Hub contains a curated collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated our submission process so you can easily add your data, too!

Using the Data Hub

If you're trying to decide which AAV is right for your experiment, searching the Data Hub's curated collection of reports (which you can filter by serotype, species, or promoter) can save you significant time and money. For instance, this report shows that anterograde, monosynaptic connections between BLA projection neurons and mPFC interneurons can be labelled with an AAV1 Cre virus combined with a Cre-dependent AAV9 tdTomato virus in the mPFC.  The AAV9 is so strong that you can even see dendritic spines! 

Curious? Watch our AAV Data Hub Challenge recording to see two reports presented by the submitting scientists, learn more about the AAV Data Hub, and hear the incredible AAV talks given by Connie Cepko and Ian Wickersham.

Submitting to the Data Hub

If you've ever validated an Addgene or CLOVER AAV, you can (and dare we say, should!) submit your data to the Data Hub! The process is easy with our new submission form - and no worries if you get interrupted by a freezer alarm going off, thanks to our handy-dandy "save" button. You can check out our step-by-step submission guide or email datahub@addgene.org to learn more.

A great submission includes:

• Images that clearly show viral spread and cellular tropism* (min. 300 dpi, PNG format)
• At least two replicates of your experiment*
• Functional data, if the report describes a functional vector such as a channelrhodopsin
• Images showing the injection site as well as the target region, if the report describes a retrograde serotype
• An abstract that summarizes your experiment clearly and concisely*

*required for a DOI.

Reports that meet our minimum standards (* above) will be given a DOI for easy citation by others and, when published, will meet FAIR data-sharing guidelines.

But what if my AAVs didn't work? 

We love negative data, and in fact, what we're most hoping to see from the community is your negative data. We want to make sure people are selecting AAVs that will work for their experiments, and a key step for that is sharing the data when an AAVs doesn't work. Sharing your negative data helps others to design better experiments (and wouldn't it be nice if those weeks and months of work was cited by others?) 

Ready to submit? 

Fantastic! The next 25 submissions will receive a 20% off coupon for a viral prep. And if you've used the Data Hub to help select an AAV for your experiments, let us know (datahub@addgene.org) and we might just send you a new Addgene Data Hub t-shirt!