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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

Posted by Various Addgenies on Dec 10, 2019 8:58:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

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Topics: Other Plasmid Tools, Plasmids

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Posted by Beth Kenkel on Nov 19, 2019 9:08:50 AM

It’s time to choose your own protein purification adventure. You want to purify your favorite protein (YFP). You have two options: 

Option #1: Affinity tag purification

You tag YFP and use an affinity column for purification. After binding YFP to the column, you wash several times to remove non-specific proteins, and then elute YFP. 

Option #2: Opto-Nanobodies (OptoNBs) purification

You skip adding a tag to YFP and instead use OptoNBs. You fill a column with OptoNB coated beads and wrap the column with blue LED lights. When you switch off the lights, OptoNBs bind YFP and non-specific proteins flow through. To elute YFP, you turn on the blue lights.

Which option do you choose? 

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Topics: Optogenetics, Other Plasmid Tools, Plasmids

FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

Posted by Guest Blogger on Oct 17, 2019 8:28:58 AM

This post was contributed by Alessia Armezzani, scientific communication manager at genOway.

A few decades ago, the brain remained elusive, not from a lack of intellectual curiosity on the part of scientists but, rather, from the limited technologies available. Over the past few years, however, remarkable technological advances have taken researchers to the threshold of a revolution in modern neuroscience, an era in which technologies like FLEx and optogenetics meet, allowing scientists to investigate the fundamental basis of both brain function and dysfunction.

First, let’s start with the basics: What is optogenetics?

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Topics: Other Plasmid Tools, Plasmids

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Posted by Various Addgenies on Sep 3, 2019 9:15:19 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

Read More >

Topics: Other Plasmid Tools, Plasmids

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Posted by Various Addgenies on Jun 6, 2019 8:41:37 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

Read More >

Topics: Other Plasmid Tools, Plasmids

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