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Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Posted by Various Addgenies on Jun 6, 2019 8:41:37 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

 

 

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Topics: Other Plasmid Tools, Plasmids

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Posted by Various Addgenies on Dec 6, 2018 8:34:06 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

Read More >

Topics: Other Plasmid Tools, Plasmids

Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

Posted by Various Addgenies on Sep 18, 2018 9:33:20 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.

Read More >

Topics: Other Plasmid Tools, Plasmids

Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Posted by Jennifer Tsang on Sep 4, 2018 8:53:28 AM

Researchers express genes of interest from plasmids in order to study gene function or to engineer cells for specific purposes. Unfortunately, plasmid copy numbers vary within cell populations and over time resulting in variable gene expression that can impact observed phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy number in a cell.

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Topics: Plasmid Elements, Other Plasmid Tools, Plasmids

RaPID Detection of RNA-protein Interactions

Posted by Beth Kenkel on Aug 16, 2018 8:40:17 AM

Sometimes it feels like DNA and protein get all the attention.There are numerous ways to detect DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect protein-protein interactions (yeast-two hybrid, Co-IP, BioID),  and that’s just to name a few. But what if you want to study RNA-protein interactions? The characterization of RNA-protein interactions has lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction detection (RaPID) method. RaPID borrows the E. coli biotin ligase BirA* from BioID and allows a researcher to identify proteins that bind an RNA motif of interest in living cells.

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Topics: Other Plasmid Tools, Plasmids

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