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RNA Interference in Plant Biology: New Tools for an Old Favorite

Posted by Guest Blogger on Oct 27, 2020 9:15:00 AM

This post was contributed by guest blogger, Robert Orr, who recently received a Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute.

What is RNAi?

The loss-of-function (LOF) experiment functions as the building block of our understanding of complex biological processes. Many tools exist to perturb biological function in a direct or unbiased way at the DNA, RNA, or protein level. The “correct” choice of tool requires careful balancing of the inherent advantages and limitations of any technique in the context of the biological question. For example, while gene knockouts have long been considered the “gold-standard” for LOF studies, the high gene copy number found in plants makes traditional knockouts unattractive from a practical perspective. Therefore, techniques that function downstream of DNA, such as RNA interference, can reversibly exert their effect independent of gene copy number.

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Topics: Plant Biology, Other Plasmid Tools, Plasmids

Hot Plasmids - October 2020

Posted by Various Addgenies on Oct 13, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

Here's what you'll find in this post:

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Topics: Hot Plasmids, Other Plasmid Tools, Plasmids

Hot Plasmids - August 2020

Posted by Various Addgenies on Aug 18, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

Here's what you'll find in this post:

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Topics: Other Plasmid Tools, Plasmids

No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

Posted by Angela Abitua on Jun 18, 2020 9:15:00 AM

Nanobodies from camelids have gotten a lot of press for being a promising approach for treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable the virus to enter host cells. To do this, they injected the spike protein from two different coronaviruses into a llama over several days and isolated special camelid antibodies that bind to the spike proteins in cell culture, and inhibit these viruses. 

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Topics: Other Plasmid Tools, Plasmids, COVID-19

Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

Posted by Various Addgenies on Jun 2, 2020 9:15:00 AM

Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here

Read More >

Topics: Hot Plasmids, Other Plasmid Tools, Plasmids

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