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Light Up Your Experiments with the Michael Davidson Collection

Posted by Lianna Swanson on Oct 31, 2017 9:22:25 AM

Michael Davidson (1950-2015) dedicated his scientific career to 3 major avenues – mentoring young students and instilling a strong work ethic in them, developing educational resources for microscopy, and building new fluorescent protein tools for the scientific community. Davidson took the fluorescent proteins originally developed by Roger Tsien, a frequent collaborator, and expanded on then to revolutionize the study of cell biology. In 2014, Mike Davidson deposited his plasmid tools with Addgene.

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Topics: Fluorescent Proteins, Generating Fusions

Fluorescent Tagging of Endogenous Genes with SapTrap

Posted by Michelle Cronin on Oct 12, 2017 10:26:13 AM

Since the discovery of GFP over 50 years ago, the growing spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function of cellular systems. FPs have been used to track protein localization, cell structure, intracellular trafficking, and protein turnover rates. Additionally, by engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular processes, including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery of many cellular mechanisms, there are some limitations to working with FPs. Overexpression of fluorescently tagged proteins can lead to improper protein localization, protein aggregation, or disruption of normal protein function, and ultimately misinterpretation of the protein’s cellular role.

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Topics: Fluorescent Proteins, Generating Fusions

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