Since the discovery of GFP over 50 years ago, the growing spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization and function of cellular systems. FPs have been used to track protein localization, cell structure, intracellular ...
In biology as in life, more is often better. More transcription factor binding sites in a promoter lead to higher transcriptional activation. Multiple nuclear localization signals (NLS) increase protein import into the nucleus. In developing their SunTag technology, the Vale and ...
High-throughput cloning, in a nutshell, is the systematic combination of different genetic sequences into plasmid DNA. In high throughput cloning techniques, although the specific sequences of the genetic elements may differ (e.g., a set of various mammalian promoters), the same ...
This post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate the power of the Dark Side." --Darth Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical "Force," this quote is equally applicable to many ...
Updated May 10, 2021. In the world of fluorescent proteins and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350 scientists from more than ...
