Antibodies 101: Normalization and Loading Controls for Western Blots

By Emily P. Bentley

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A cartoon of a prime editor with two different edit sequences. The DNA sequences are shown with one strand edited and a 5′ DNA flap, before heteroduplex resolution and DNA repair.  The first edit has an unchanged PAM. This DNA is shown connected to the prime editor by a two-way arrow, indicating that the editor can re-bind. Re-nicking is represented by scissors and would remove the newly edited DNA.  The second edit has an altered PAM. A one-way arrow leads from the prime editor to this edit, indicating that the changed PAM prevents the editor from re-binding.
The Codon Guide to Synthetic Biology logo
Triplicate samples laid out in a 96 well plate
Screenshot of Intro to Lab Bench homepage

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