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The time and cost required to make a plasmid

Posted by Jennifer Tsang on Sep 11, 2018 9:48:42 AM

Have you ever wondered how long it takes to make a plasmid? Or have you thought about how much total time you spend in the lab cloning before you can start on your experiment? What about all the reagents you need to order? Sometimes, it feels like an eternity of cloning, waiting, and repeating before you can finally dive deep into experiments.

Since one million plasmids shared is upon us at Addgene, we started wondering, just how much time and money do researchers spend cloning? How much does material sharing speed science?

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Topics: Plasmid Cloning, Lab Tips

Overcoming the challenges of lentiviral production

Posted by Meghan Rego on Aug 7, 2018 8:31:12 AM

While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges. Whether choosing a system that is the best fit for the experiment, trying to produce virus of a usable titer, or fine-tuning selection and expression in your target cell line, researchers often find themselves faced with a roadblock. In this post, we will provide an overview of some of the common challenges associated with producing and using lentivirus and offer some tips and tricks for overcoming these hurdles.

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Topics: Viral Vectors, Techniques, Lab Tips

10 Basic tips for mammalian cell culture

Posted by Guest Blogger on Jul 12, 2018 9:09:21 AM

This guest post was contributed by Sana Khan Khilji, a PhD student at the Max Planck Institute of Colloids and Interfaces.

Here are some tips for cell culture that will hopefully help you keep a well organized lab and contamination free environment for successful experiments:

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Topics: Lab Tips

Replacing paper: tips for choosing an electronic lab notebook

Posted by Guest Blogger on Jul 10, 2018 9:08:54 AM

This post was contributed by guest blogger, Tea Pavlek, Product Marketing Manager at sciNote.

Today, every lab has its own habits and approaches to record keeping. Top priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes, the lab's success and the careers of its researchers are on the line.

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Topics: Reproducibility, Open Science, Lab Tips, Lab Software

Lab Automation at Addgene

Posted by Shannon Rinaldi on Mar 22, 2018 9:28:38 AM

If you’re doing high throughput work, it’s likely that you come into the lab wishing there was a machine that could make your life a hundred times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots that’ll take a little labor off our hands.

Good news! There is probably a machine or robot out there that does exactly what you want plus way more than you thought it could ever do! However, there is quite a bit of upfront research you have to do to find said robot and to find out how you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG). There is so much to see and so many more robots than you ever knew existed!

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Topics: Inside Addgene, Lab Tips

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