Plasmids 101: Fluorescent Biosensors

New Podcast Segment: Hot Plasmids

Retrograde AAV: Making the Journey from Axon to Nucleus

Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Multiplex Genome Editing with CRISPR-Cpf1

Plasmids 101: Fluorescent Protein Timers

How to Design Your gRNA for CRISPR Genome Editing

Tips for Getting a Faculty Position

SciComm with the Experts at Science in the News Part 2

Plasmids 101: Photoactivatable Fluorescent Proteins

March for Science

Addgene Depositors Get More Citations

The Power Behind NGS Plasmid Validation: seqWell

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Plasmids 101: Aptamer Fluorophores

Plasmids for Endogenous Gene Tagging in Human Cells

Screening for Successful Genome Editing with Digital PCR

Plasmids 101: SunTag and Fluorescent Imaging

Addgene’s Viral Service - Why Virus? Why Now?

Quick Guide to All Things Lentivirus

SciComm with the Experts at Science in the News Part 1

Cancer and the Immune System: Deciphering the Relationship

What Do I Do Now? Academic v. Non-Academic Career Decisions

Quick New Way to Deposit Plasmids: The Deposit Spreadsheet

Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

Addgene Moves to NGS Verification Powered by seqWell

CRISPRainbow and Genome Visualization

Science Career Options

Deep Mutational Scanning with One Pot Saturation Mutagenesis

How to Write a Scientific Review Article

CRISPR 101: Editing the Epigenome

5 Science Rap Videos & Their Creator: Tom McFadden

MXS Chaining

Plasmids 101: Environmental Plasmids

Plasmids: Simplified

Plasmids 101: Gateway Cloning

Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Truncated gRNAs for Regulating Gene Expression

Top Requested Lentivirus and AAV of 2016

Michael Davidson and Roger Tsien Commemorative Travel Awards

27 Hot Plasmids from 2016

Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

Mesothelioma - Causes, Symptoms, and Treatment

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

With Our New Viral Service, We're Taking CRISPR Further!

'Tis the Season to #DeckTheLab

Plasmids 101: Knockout/Knock-In Plasmids

Bricking Science: Portraying Scientific Reality Through LEGO

Tips for Improving Your Next Manuscript

10 Great Guest Posts We're Thankful For

Using Addgene's New Viral Service for Your Research

Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

Antibiotic Resistance: An Old Solution but a New Problem

Plasmid Grant to Aid Cervical Cancer Screening in Ghana

Editor's Choice, October 2016

Using Video to Share Your Science: We Share Science

FastCloning: A Newer, Simpler Cloning Technique

Plasmids 101: TOPO Cloning

Changing Labor Laws Bring Increased Postdoc Wages

5 Reasons to Use Reddit for Science Communication

6 Tips for Grant Writing

Healthcare Consulting: A Door to the Business of Life Sciences

CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

Editor's Choice, September 2016

Grad School Advice Part 2: Building Community

Sequencing Options for CRISPR Genotyping

Technique: Probe Phage Genomes for Host Binding Proteins

Four Factors that Differentiate the Stem Cell Field

Tips for Writing a Good Cover Letter

5 Great Apps for Lab Life

Tips for Technical Support Calls

Running for Rare Disease, Running for FOP, Running for AJ

Multiple Plasmids at a Low Price: Kits from Addgene

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Addgene's Cancer Collection Pages Connect the Research Community

Editor's Choice, August 2016

Grad School Advice Part 1: Picking a Lab and a Project

Adapting Toehold Switches to Detect Zika Virus

Developing Lab Management Software for Biology

10 Steps to a Perfect Science Talk

Cas9 Activators: A Practical Guide

Single Base Editing with CRISPR

5 Tips for Troubleshooting Viral Transductions

CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

Editor's Choice, July 2016

Google Forums Round Up: First Impressions of NgAgo

Site Directed Mutagenesis by PCR

Recombinase-based State Machines Enable Order-dependent Logic in vivo

AAV: A Versatile Viral Tool for Gene Expression in Mammals

Gendered Innovations: Why Does Sex of the Cell Matter?

Special Delivery: Fluorophore Targeting for FRET Studies

Cpf1 Update: Comparison to Cas9 and NgAgo

Generating Mouse Models Using CRISPR/Cas9

Addgene at the ASM Microbe 2016 Conference

Plasmids 101: Methylation and Restriction Enzymes

Tips for CRISPR Gene Editing in Mice

Using Phosphoserine to Study Protein Phosphorylation

Evolution of Lab Techniques

Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

Introducing Addgene's Microbiology Pages

Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

Using Virus in Your Research - A Primer for Beginners

How to Keep a Lab Notebook for Bioinformatic Analyses

PiggyBac-ing Through the Genome Editing Field

Plasmids 101: Optimizing Plasmid Yields

Podcast: A Malawian Professor's Path to Biotech Research

Lentiviral Vector Uses and Overview

Searchable and Sortable gRNAs for Your Next CRISPR Experiment

Plasmids 101: Colony PCR

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

The Challenges of Cell Culture

CRISPR Antimicrobials

Plasmids 101: FLEx Vectors

Casilio: An Adaptive, Multitasking “CRISPR-OS”

Genome engineering using Cas9/gRNA Ribonucleoproteins (RNPs)

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

R Bodies: Membrane-Rupturing Microscopic Tools

Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

Getting the Most from Your Lentiviral Transduction

Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

Plasmids 101: Terminators and PolyA signals

Plasmid Cloning by PCR

Optimizing Donor DNA for Enhanced CRISPR Genome Editing

Modulate the Activity of 17 Signaling Pathways with One Kit!

Minigenomes - a Safe Way to Study Dangerous Viruses

Suggest A Plasmid You'd Like to Find at Addgene

Tips for Titering Your Lentiviral Preps

Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

Optogenetics + CRISPR, Using Light to Control Genome Editing

CRISPR Methods for Bacterial Genome Engineering

Call for Guest Bloggers

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Experimenting with New Careers while in Grad School

PITChing MMEJ as an Alternative Route for Gene Editing

CRISPR-Cas9: Tips for Optimizing sgRNA Activity

Plasmids 101: Restriction Cloning

REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

How to Lead a Great Meeting

Anatomy of a Plasmid Page at Addgene

Components of CRISPR/Cas9

CRISPR 101: Multiplex expression of gRNAs

Treating Muscular Dystrophy with CRISPR Gene Editing

Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Addgene's Tips for Plasmid Quality Control

Finding Your Perfect Job After University

How to Deposit Your Plasmids with Addgene

Your Top Requested Plasmid in 2015!

28 Hot Plasmid Technologies from 2015

Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Enhancing CRISPR Targeting Specificity with eSpCas9 and SpCas9-HF1

A Better Way to Get Customer Support: The Help Center

An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

Addgene's Top 10 Blog Posts of 2015

Plasmids 101: Stringent Regulation of Replication

Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

#DeckTheLab to Win a Tasty Winter Treat This Holiday Season

Thoughts on the Future of Research 2015 Boston Symposium

Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

Illuminating Epigenetics with A FRET Based Biosensor

Networking and Inspiring Young Scientists at Freshers Fairs

MassBioEd Career Exploration Day at Addgene

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Inntags: Innovative Protein Epitope Tagging

Building Global Connections with the International Mentorship Program USA-EUROPE

The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

Plasmids 101: A Brief History of Plasmids and an Improved eBook!

Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

Tips for Screening with Yeast Two Hybrid Systems

The Future of Research Symposium Boston 2015

Cpf1: A New Tool for CRISPR Genome Editing

Tips from the Repository Trenches: Using Barcodes to Track Samples

Important Considerations in Optogenetics Behavioral Experiments

pSiM24: Simplifying Plant Genetic Engineering

CRISPR 101: Mammalian Expression Systems and Delivery Methods

New Tool for Lineage Tracing: The ClonTracer Library

The Materials Science of Optogenetics Experiments

Donations from Addgene to Yield Answers for Rare Disease Researchers

A Primer on Optogenetics: Introduction and Opsin Delivery

Synthetic Photobiology: Optogenetics for E. coli

The Importance of a Fun Workplace: Company Culture at Addgene

How to Negotiate a Successful Lab Start Up Budget

Plasmids 101: Golden Gate Cloning

Don’t Wait To Launch Your Health Science Startup

A Career in Grants: How To Become a Grant Professional

Genome-wide Screening Using CRISPR/Cas9

Addgene Welcomes John Doench to the Advisory Board

Mapping the 4D nucleome with CRISPR/Cas9

Gaining Leadership Skills Volunteering at a Professional Organization

Seeing Red: Simple GFP Photoconversion

CRISPR 101: Validating Your Genome Edit

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Experimenting in an Open Source lab: from CRISPR to Cats

IDP and your PI: A Roadmap for Career Planning and Personal Development

Light Sheet Fluorescence Microscopy

A Match Made in Heaven: CRISPR/Cas9 and AAV

6 Steps to Submitting a Resume That Gets Seen

Even more elegant: Single injection CRISPR/Cas9 in C. elegans

Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

Sleeping Beauty Awakens for Genome Engineering

Another Pathway into Cells: iTOP

Easing the Protein Purification Process with pCri

Science Communication Snapshot: DayCon 2015

More Data for You: Find Articles Citing Addgene Plasmids

Plasmids 101: Blue-white Screening

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

6 Tools & Tips: Online Social Networking for Scientists

Cre-ating New Methods for Site-specific Recombination in Drosophila

Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

CRISPR 101: Non-Homologous End Joining

Plasmids 101: Control Plasmids

Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

Management for Scientists: Managing vs. Leading

500,000 Plasmids Shipped and Counting!

CRISPR 101: Homology Directed Repair

The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

CRISPR 101: A New Series on Genome Editing & CRISPR-Cas

Science as Art

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Management for Scientists: Delegating is Key

CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

Plasmids 101: E. coli Strains for Protein Expression

Management for Scientists: Giving Feedback

Trends in CRISPR and SynBio Technologies [Slideshare]

Career Coaching for Scientists: Why and Where Do I Find One?

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

Plasmids 101: Cre-lox

Management for Scientists: Seeking Feedback

22 Hot Plasmid Technologies from 2014

Resume Writing for Non-academic Science Careers

Addgene’s Top 10 Blog Posts from 2014

Plasmids 101: Protein tags

Management for Scientists: What Makes a Good Manager Anyway?

Make a Splash: Notions of Scientific Impact Are Evolving

Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

How Exercise Purges Stress and Keeps Depression at Bay

We've Launched Addgene's New & Improved Website!

Fueled by Coffee at #SfN14

Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering

Plasmids 101: Common Lab E. coli Strains

Tips for Using FRET in Your Experiments

Countdown to Halloween @Addgene

How-to: 5 Steps to a Great Panel Discussion

A Scientist's Guide to Ebola

Choosing Your Fluorescent Proteins for Multi-Color Imaging

Future of Research Conference - Remarkable Opening Session

ICYMI: Addgene’s Plasmids 101 eBook

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

Plasmids 101: Multicistronic Vectors

A Conference By Postdocs For Postdocs: Future of Research

Plasmids 101: How to Verify Your Plasmid

Advice for Moving Into Sales After Your Science Postdoc

Choosing Your Perfect Empty Backbone

SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

How to Make Friends and Meet People at a Scientific Conference

Data Freedom: The Expansion of Data Sharing in Research Publications

The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

3 Challenges in Plant Synthetic Biology

Plasmids 101: Viral Vector Elements

Working with Nuclear Receptors

Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Synthetic Biology & the Importance of Assembly Standards

Inside Addgene's 10 Year Anniversary Gala

Scientist Networking: What is an Informational Interview?

6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

Plasmids 101: Luciferase

Designer PUF Proteins for Any RNA Target

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

London Calling - Addgene’s New Europe Office in London

Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

Developing Transferable Skills During Science Training

Tips for Using BLAST to Verify Plasmids

Dos & Don'ts When Publishing a Scientific Manuscript

Which Fluorescent Protein Should I Use?

Plasmids 101: Green Fluorescent Protein (GFP)

10 Ways to Share Your Science!

Celebrating Accomplishments in the Lab

Hot Plasmids: FRET-Based Biosensors

Why Do I Need an MTA Anyway?

Advice for Scientists Starting a Lab

Your Lentiviral Plasmid FAQs Answered

Advice for Choosing a Research Project

The Stingy Scientist: How the Baby Gel Box Was Born

Finding and Joining Your Dream Lab

Plasmids 101: The Promoter Region – Let's Go!

9 Tips to Achieve Success in Academia

In Living Color: The Skinny on In Vivo Imaging Tools

Plasmids 101: Mammalian Vectors

Negotiating Work and Life: How to Find the Joy

CRISPR-Cas9 FAQs Answered!

Keeping Up With CRISPR/Cas9

Mentoring for Scientists: I Have a Mentor, What Now?

Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

Why Scientists Should Give Twitter a Try

Plasmids 101: Yeast Vectors

Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

5 Steps to Writing Grant Proposals for Grad Students

Plasmids 101: Origin of Replication

Will You Be My Mentor? Finding and Asking for Mentoring Support

Plasmids 101: Antibiotic Resistance Genes

Save Time and Money by Making Your Own Competent Cells

Quick, Versatile Plant Transgenesis with GreenGate Plasmids

Extracurricular Activities for a Strong Science Career Path

Choosing a Good Mentor for Scientists

Plasmids 101: What is a plasmid?

The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

Using CRISPR/Cas9 to Edit Disease Out of the Genome

Three Tips to Organize Your Lab Notebooks in the New Year

The Addgene Zoo - A Plasmid Repository with a Diverse Collection of Genes

Top 10 Open Science Developments of 2013

"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Kiran Musunuru on the Newest TALEN Genome-Editing System

Overwhelmed? Take a Break With Our 5 Favorite Science Comics

From our Table to Yours: An Inside Look at Lunch at Addgene

History of CRISPR Cas - A tale of survival and evolution

Let There Be LITE Plasmids

Tag Your Favorite Yeast Genes with Ease

Addgene’s New Way to Help You Share Science

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