A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

By Jennifer Tsang

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Schematic for transforming pEcgRNA and pEcCas into E. coli to edit the genome. Plasmids are subsequently cured from the cell.
Multiplexed insertion is possible using a single plasmid that contains a multi-spacer CRISPR array. This encodes separate gRNAs that target different locations in the genome.
Metagenomic Alteration of Gut microbiome by In situ conjugation addgene blog
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Acoustic reporter genes graphical abstract
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