By Guest Blogger
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If you’ve ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for designing your overhang sets. But are those rules the best way to design GGA overhang sets, particularly for high-complexity reactions?
The Retroviridae (commonly called retrovirus) family — of which HIV is a member — may seem like an unlikely candidate to use as a viral vector, but retroviruses have been developed into some of the most widely used tools in molecular biology. “Retroviruses” is an umbrella term ...
For safety and production reasons, multiple plasmids with many components are used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc.) and ...
Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has substantial expansions on the chalk talks and Zoom interviews sections, along with new thoughts on research statements. Snapp, who is always looking for ways to improve his eBook, ...
If you’ve ordered a pooled library from Addgene, you may have been a little surprised at how this powerful tool is in such a small package. But don’t let the simple presentation deceive you; pooled libraries can be quite challenging to use! In this post, I’ll walk you through ...
In order to understand the complex wiring of the brain and the underpinnings of neurological disease, neuroscientists need to be able to probe cells and circuits without disrupting normal brain function. Chemogenetics has become a popular tool in the field as it provides a ...
The calcium is strong here….I can sense it….sound like a plot to an biology scifi movie? Or maybe it’s a biosensor?! You can do more than just ‘sense’ biological compounds and reactions; you can quantify them with biosensors! In this blog we will review the basics of biosensors ...
