Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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If you’re interested in studying immunology or subpopulations of cells, you’ll soon find yourself encountering flow data in the literature. Data reported from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. But in order to plan ...
Addgene is excited to announce we now offer an export license application for rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled materials in the USA, where Addgene is located. The export license, if granted, allows us to ...
Cellular communication and function depend on potential energy. Cellular activities like action potentials, muscle contractions, and the control of voltage gated ion channels all depend on transmitting and sensing electrical potential. Thankfully, there is a tool to measure ...
Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward a decade, and the CRISPR field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it can ...
With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity to once again reflect on our achievements in the last year and share the exciting things to come in the new year. I am proud of what we accomplished in 2023 and thrilled to ...
¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español!
Annotation of genes in immune cells typically involves the creation of germline knockout mice, which is time-consuming, as it only changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells in a high-throughput manner, but these systems have not been ...
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