Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Getting ready for Society for Neuroscience Conference 2023? So are we! Come say hi to us at Addgene's booth, #3422, in the nonprofits section.
There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field and the greater scientific community. In 2014, we wrote a blog post on CRISPR’s potential for correcting monogenetic diseases. Now, almost 10 years later, CRISPR’s potential ...
Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies."
Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through how we produce both our AAV preps and our lentiviral preps.
Adeno-associated viruses (AAV) are an important biological tool that allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important elements, but among the most important are the inverted terminal repeats (ITRs). ITRs are the ...
The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from crossing into the brain. While helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally, delivery is achieved through the use of ...
Plasmids need antibiotic resistance genes so that you can specifically isolate bacteria containing your constructs of interest. But does it matter which antibiotic resistance gene you select? In this blog, we’ll break down the mechanism of action of the most common antibiotic ...
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