Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
Subscribe
If you’re interested in learning a new skill, getting access to equipment you don’t have, or maybe both, a visiting scientist stint may be ideal for you. Here, we will chat about who might want to explore this role, how to seek out such an arrangement, and how to operate once in ...
Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can’t even decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact — and not just on the disease front.
Calcium is arguably one of the most important metal ions in biology (sodium and potassium are up there too!). Calcium is fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural component of bones and teeth. It is an enzyme ...
Flow cytometry is one of the most powerful tools available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue type, such as PBMCs or tumors, and moreso than other methods, the identification and isolation of rare cell types. In ...
If you’ve ever used Golden Gate Assembly for cloning, you might be familiar with the rules of thumb for designing your overhang sets. But are those rules the best way to design GGA overhang sets, particularly for high-complexity reactions?
The Retroviridae (commonly called retrovirus) family — of which HIV is a member — may seem like an unlikely candidate to use as a viral vector, but retroviruses have been developed into some of the most widely used tools in molecular biology. “Retroviruses” is an umbrella term ...
For safety and production reasons, multiple plasmids with many components are used to package lab grown viruses. It can be intimidating at first to look at so many plasmid maps and protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc.) and ...
.png)
