Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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Schematic of SEND system
Schematic for transforming pEcgRNA and pEcCas into E. coli to edit the genome. Plasmids are subsequently cured from the cell.
CRISPR cheat sheet
Addgene presents... the 12 days of CRISPR #12DaysOfCRISPR
Multiplexed insertion is possible using a single plasmid that contains a multi-spacer CRISPR array. This encodes separate gRNAs that target different locations in the genome.

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