By Rachel Leeson
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CRISPR is a sleek acronym for a real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing tool… but ...
Prime editing is a versatile genome editing technology that allows precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019; Chen & Liu, 2023). This method uses a prime editor (typically ...
Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’s so important to choose a target sequence with a nearby protospacer-adjacent motif (PAM)? Cas9 is becoming an ever more ubiquitous tool for genome engineering, and studying its ...
FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However, in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine microenvironment that exists in vivo. Over the past five years, in vivo ...
As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on science and scientists around the globe. Our first story focuses on a technology that has changed virtually all of biology…CRISPR.
The PAM… that sneaky little bit of sequence that you hope is present next to the "perfect" guide sequence for your genome engineering experiment. With CRISPR entering the clinic for correction of disease-causing alleles, and the growing need for gene editing in research, the old ...
Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward a decade, and the CRISPR field has exploded with dozens of Cas enzymes and variants available. Without a comprehensive resource, it can ...
