By Tyler Ford
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🎶 On the 12 days of CRISPR, Addgene gave to me… 🎶 A few years ago we used the #12DaysOfCRIPSR hashtag to share new CRISPR content designed to help you with your CRISPR experiments and to learn about new CRISPR tools. Since the CRISPR field keeps changing, we’ve been updating ...
Originally published Jan 27, 2015 and last updated Dec 17, 2020. This post was contributed by Jordan Ward who is an assistant professor at the University of California, Santa Cruz. Emerging CRISPR/Cas9 editing technologies have transformed the palette of experiments possible in ...
Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel. Adenoviral vectors (AdVs) have been used for many years for in vivo delivery and gene therapy and represent one of the most studied viruses. Adenoviruses are non-enveloped double-strand DNA viruses ...
This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA transposons are ubiquitous genetic elements capable of spreading within and between genomes, and have been adapted for a wide variety of ...
Prior to the discovery of CRISPR/Cas systems, gene activation across multiple loci was an arduous process. When using zinc finger proteins or TALE proteins, proteins had to be re-engineered for each gene, making wide-scale gene activation seem next to impossible. The development ...
Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems were first discovered in bacteria, most CRISPR-based genome engineering has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double ...
Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific DNA ...
