The 12 Days of CRISPR: 2021

By Rachel Leeson

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Cartoon depiction of a superhero cutting a chain tethering TET1 to dCas9
Schematic of SEND system
Schematic for transforming pEcgRNA and pEcCas into E. coli to edit the genome. Plasmids are subsequently cured from the cell.
CRISPR cheat sheet
Addgene presents... the 12 days of CRISPR #12DaysOfCRISPR
Using CRISPR in C. elegans to knock-in FLAG tag.
 Schematic of targeted gene knock-out (left) and targeted DNA knock-in (right) by CRISPR/Cas9. Adenoviral CRISPR vectors (left) or CRISPR vectors and donor DNA vectors (right) are transduced to target cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final step of targeted gene knock-out (left), a fragment is deleted from two homologous strands of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA knock-in, donor DNA is copied into the cut site through error-free homologous recombination (HR).

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