This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed your gRNA and introduced it into your target cells with Cas9. Hooray! Now it’s time to make sure your genome edits went according to plan. In this blog post we’ll explain ...
This post was originally written by Joel McDade and significantly updated in 2022 by Susanna Stroik. The advent of CRISPR/Cas9 has made it easier than ever to make precise, targeted genome modifications. Cas9 has been modified to enable researchers to knock out, knock in, base ...
The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to target the genome of the invading phage pathogen. Over the past decade, CRISPR tools have leveraged such host-defense mechanisms to enable multiplex gene editing in a variety of ...
This post was originally written in 2014 by Kendall Morgan and updated in 2022 by Lucie Wilson. Lucie is an Addgene co-op from Northeastern University. There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field. It has the possibility ...
The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. In molecular and cellular biology, this happens through the development of tools that exploit and expand our current knowledge. The Abudayyeh-Gootenberg lab (also called the ...
One of our favorite holiday traditions is our annual Twelve Days of CRISPR campaign! But if you missed our tweets featuring the best of CRISPR in 2021 and useful resources, fear not! You can find all of them here.
This post was contributed by guest blogger Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some enzymatic fusions are godsends. Tethering dCas9 to the histone acetyltransferase p300 or the DNA methyltransferase DNMT3A allows ...
It’s time for another edition of “What’s New in CRISPR” - where we highlight a few of the newest CRISPR plasmids available at Addgene. If you want to read more content related to CRISPR technology, subscribe to our CRISPR blog content. In this post we will cover: Miniature ...
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