Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland. After completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech, Crystal moved on to a Postdoc in the chemistry department at MIT. Crystal now ...
This series was written for selfish reasons: I wanted to learn about careers in science communication. When I started my Science Communication Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest blog pieces. Throughout my ...
Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study of the brain and spinal cord, which together are known as the central nervous system (CNS). AAVs are popular tools because: 1) their genomes are easy to manipulate, 2) they have ...
This post was contributed by guest blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory. Are you a science student or early career researcher looking to break into science communication? Everybody goes about this in their own way. The career paths into science ...
This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although we only walk one path at a time, the variety of paths in life is mind-boggling. Unlike a mountain – with many routes up and only one destination – a career in the sciences ...
This post was updated on Jul 27, 2020. CRISPR, and specifically Cas9 from S. pyogenes (SpCas9), is truly an exceptional genome engineering tool. It is easy to use, functional in most species, and has many applications. That said, SpCas9 is not the only game in town, and other ...
Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with light-inducible photosensitizers and a quick zap of the proper color light, you can do just that. Photosensitizing dyes and proteins have been around for awhile (check out this ...
