This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ...
This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. In this second post in our quick guide to working with Drosophila, you’ll learn how to maniupate expression of your favorite gene (YFG) in flies. Read the first post here. Once ...
This post was contributed by guest blogger Jon Chow, an immunology PhD student at Harvard University. Do you have a gene of interest but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you find an ortholog in the Drosophila ...
Cre-lox recombination is an incredibly useful molecular biology tool, but like any biological system, it has certain drawbacks. First, the efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce recombination at pseudo- or cryptic ...
