Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often describe a process that begins with cell lysis to release AAV ...
This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. Are you currently on or planning to go on the academic job market? In addition to all of the documents you submit, ...
New ideas and approaches are encouraged in science. It isn’t surprising that new ideas are also popping up in how we manage lab waste. This is the second in a series of posts on bringing sustainable practices in the lab. The first post focused on streaming some lab waste for ...
Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This type of cell labeling technique involves the introduction of a transgene construct containing fluorescent proteins (XFP) of different colors to label an organ or entire ...
In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research. But he realized that it wasn’t enough to discuss the age-old problem of irreproducibility in science or even to discuss potential solutions. Despite all the talk, not ...
Career and skills development can happen anywhere, whether you realize it or not. In the lab, you could be managing a team, planning meetings, and finding the best way to organize your time. At a conference, you could be honing your science communications skills and networking. ...
Mutagenesis is a tool that both evolution and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify and/or facilitate the evolution of new phenotypes, and forward genetics harnesses this at a large scale by screening diverse ...
