Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find new CRISPR tools for your research.
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often describe a process that begins with cell lysis to release AAV ...
This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute. Are you currently on or planning to go on the academic job market? In addition to all of the documents you submit, ...
New ideas and approaches are encouraged in science. It isn’t surprising that new ideas are also popping up in how we manage lab waste. This is the second in a series of posts on bringing sustainable practices in the lab. The first post focused on streaming some lab waste for ...
Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This type of cell labeling technique involves the introduction of a transgene construct containing fluorescent proteins (XFP) of different colors to label an organ or entire ...
In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in research. But he realized that it wasn’t enough to discuss the age-old problem of irreproducibility in science or even to discuss potential solutions. Despite all the talk, not ...
