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R Bodies: Membrane-Rupturing Microscopic Tools

Posted by Guest Blogger on Apr 14, 2016 10:30:00 AM

This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela Silver. 

Most types of biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced by orchestrated movements of networks of proteins consuming molecular fuel sources. While the importance of understanding these complex processes can’t be overstated, we can also learn a lot from Nature’s simpler solutions to transmitting forces over long distances. For instance, how much force can be generated by conformational changes in proteins? How can information propagate through a structured material over a long distance? And can we understand such a structure well enough to engineer it to suit our purposes?

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Topics: Plasmid Technology, Synthetic Biology

Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

Posted by Guest Blogger on Apr 12, 2016 10:30:00 AM

This post was contributed by guest blogger Alan Wong.

The complexity of biological systems can hinder our attempts to study and engineer them, but what if we had a simple tool that allowed us to rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal of providing an easy-to-use tool to analyze the complex combinatorial genetic networks underlying your favorite biological phenotype in a scalable way. This blog post will introduce you to this new technology, and guide you through the basics of CombiGEM-CRISPR experiments.

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Topics: Plasmid Technology, Genome Engineering, CRISPR

Getting the Most from Your Lentiviral Transduction

Posted by Meghan Rego on Apr 7, 2016 10:30:00 AM

Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express a gene of interest. While the general approach for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting the most out of your lentiviral transduction experiments.

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Topics: Lab Tips, Viral Vectors

Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

Posted by Tyler Ford on Apr 5, 2016 10:30:00 AM

Researchers in developing countries often find themselves on the front lines of emerging global challenges. However, these same researchers face significant barriers to scientific discovery and career development. They are working with limited resources but limitless potential. We see it as part of our mission to enable all researchers and are proud to announce that we are teaming up with Seeding Labs to better serve those in developing countries.

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Topics: Scientific Sharing, Inside Addgene, Seeding Labs

Plasmids 101: Terminators and PolyA signals

Posted by Julian Taylor-Parker on Mar 31, 2016 10:30:00 AM

Plasmids designed to express genes in a given host cell type are generally broken down into two broad categories, prokaryotic or eukaryotic, based on the functional elements they contain. Plasmid DNA in both prokaryotic and eukaryotic systems must be transcribed into RNA, which occurs in three phases: initiation, elongation, and termination. In a previous post we discussed the promoter's role in the initiation step of gene transcription; today we'll provide an overview on how transcription stops, or termination. Read on to learn more!

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Topics: Plasmid Elements, Plasmids 101

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