Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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The catalytically dead Cas9 protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has been widely adapted for a wide variety of applications: base editing, CRISPR activation and inhibition, among others. Over the past few years, dCas9 ...
This post was contributed by guest blogger Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive Brain was founded in early 2018 with the goal of communicating science to non-scientists. Chinmaya Sadangi, a postdoc at the University of Toronto, created The ...
By Alyssa Cecchetelli and Lukas Morgan Model organisms are vital tools used by researchers around the globe. These organisms share many genes with humans, are easily maintained in the lab, and have short generation times that make it easy to study the effects of genetic ...
This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of Molecular Cytology and van Leeuwenhoek Centre for Advanced Microscopy (University of Amsterdam). Tagging a protein of interest with a fluorescent protein to study its function is ...
The vast majority of bacteria are undomesticated which limits the tools scientists can use to study them. For example, gene knockdown with CRISPR interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce CRISPRi machinery into ...
Addgene recently moved to our brand new location, with more space to grow and help scientists share resources in new ways. Along with a new location comes the need for new conference room names. The Addgenie’s favorite conference room naming scheme? Gene names! This is not ...
Since the beginning of our viral service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of the AAV we have released so far in 2019. You can also browse our entire AAV inventory! Our new AAVs include: ...
