Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
Subscribe
As with DNA isolation, scientists commonly rely on RNA isolation kits to make their life easier. Recently, we published a blog on DNA purification without a kit that outlined several reasons why doing something without a kit has advantages: less plastic waste, less expense, and ...
Are you a grad student, postdoc, or bench scientist who began working remotely recently? Most of us at Addgene began working from home last week to flatten the curve, but working remotely can be more difficult if your work is based in the lab. If you’re unsure what to do during ...
David Liu’s lab created the first base editor in 2016 (Komor et al., 2016) and since then has been trying to expand their precision editing capabilities. Base editors make specific DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused ...
Adeno-associated virus (AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure your gene goes where you want it to? Knowing that a gene is expressed in a particular cell type is important not only for translational research, such as gene therapy, but ...
In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene. We will still periodically focus on specific CRISPR plasmid tools more in-depth, but we hope that this blog series will help you find more new CRISPR tools for your research! ...
We’re big fans of all forms of research sharing and with Open Data Day 2020 coming up on March 7th, what better way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot in 2019 as an open science platform for scientists using Addgene AAV viral ...
Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like ...
