By Mary Gearing
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If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to begin the search is by using selection strategies, where only cells that have gained ...
Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple ...
The Rare Genomics Institute BeHEARD (Helping Empower and Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is our fourth year working with the Rare Genomics Institute by providing plasmid-based tools for rare disease research. ...
This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded version by Nyla Naim was published Sept 3, 2020. Scientists around the world have been making major improvements to CRISPR technology since its initial applications for ...
Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging capacity is limited to ~4.7kb, or roughly half the packaging limits of lentiviral and adenoviral vectors. While many transgene will fit within this limit, some like prime ...
This post was contributed by Patrick Miller-Rhodes from the University of Rochester Medical Center. You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the ...
Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is unique compared to the rest of the genome and (2) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM ...
