Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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This post was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical Center. DNA transposons are ubiquitous genetic elements capable of spreading within and between genomes, and have been adapted for a wide variety of ...
Your next cool experiment requires some AAV. Where do you start? Plasmids of course! You just need three plasmids to start making the AAVs you need for your experiment: the packaging plasmid which contains the AAV structural and packaging genes, the adenoviral helper plasmid ...
This post was contributed by guest blogger, Rachel Rubinstein, a field-based software product manager at Oxford Nanopore Technologies. If the first thing you hear when someone says they’re a software product manager is “project manager,” you’re not alone. A few years ago when I ...
The Rett Syndrome Research Trust (RSRT) has an ambitious goal of curing Rett Syndrome and Addgene is thrilled to partner with them to launch a Rett Syndrome Plasmid Collection and Resource Center for Rett Syndrome research. With a shared passion for facilitating research and ...
Mark your calendars for November 16-20, 2020 for #DepositorWeek on Twitter! That’s today through Friday. There will be trivia, prizes, and more! We wanted to take a week to celebrate the scientists who make a big impact in open science. Depositors consistently contribute to our ...
There are 64 different codons that encode 20 amino acids and three stop codons, meaning that the same amino acid can be encoded by more than one codon. Although the genetic code is universal, many different organisms actually prefer certain codons over others for certain amino ...
Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another basic element of any plasmid: the origin of replication/replicon. The replicon is comprised of the origin of replication (ori) and all ...
