Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Originally published Mar 3, 2016 by Mary Gearing and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems were first discovered in bacteria, most CRISPR-based genome engineering has taken place in other organisms. In many bacteria, unlike other organisms, ...
Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory Board member, and Institute Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific DNA ...
This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration. “But, seriously, what even is science policy?” I have been asked this many times throughout my short science policy career and this seemingly simple ...
These are stormy times. It feels like we’re being buffeted by wind and waves and sometimes wonder if we can take another blow. Back in March, we never expected to be in this here and now. Our closure was earlier than most companies. Since the start of this crisis, we’ve been ...
Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel. CRISPR genome editing has quickly become a popular system for in vitro and germline genome editing, but in vivo gene editing approaches have been limited by problems with Cas9 delivery. ...
If you’re cloning a plasmid, you’ll need a way to find the needle in the haystack: the one perfect clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to begin the search is by using selection strategies, where only cells that have gained ...
Originally published Jan 28, 2016 and last updated Sep 10, 2020 by Jennifer Tsang. CRISPR makes it easy to target multiple loci - a concept called multiplexing. Since CRISPR is such a robust system, editing or labeling efficiency doesn’t usually change when you add multiple ...
