Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Turn it on? Turn it off? Turn it down, but not forever? You don’t need to resort to the force to control protein expression in mammalian cells: easy to use biological tools are abundant! In this post, we will review tried-and-true protein expression control tools as well as ...
When Karl Barry Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth repeat Nobel Prize winner in history. Interestingly, he also adds to the disproportionately large number of honorees to have trained under a previous ...
We're excited to roll out the redesigned, revamped, and re-launched Addgene AAV Data Hub, the place for scientists to share their AAV validation data. The Data Hub contains a curated collection of community submissions for Addgene and CLOVER AAV experiments - and we've updated ...
Transcription, translation, and prone to degradation – those are the words that describe RNA! Double stranded? Well, that’s just for DNA – right?
Every few months we highlight a subset of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) here.
Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul 27, 2020; updated by Susanna Stroik January 24, 2023. DNA damage drives genome instability and contributes to cancer, premature aging, and other harmful processes. The most ...
What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used to study gene expression in cells. But these reporters have limited use in vivo because they are based on light which is very easily scattered by tissue. Ultrasound, on the ...
