CRISPR 101: Cas9 vs. The Other Cas(s)

By Susanna Stroik

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Schematic showing MTRIA biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled receptors
Histogram of the frequency of SNPs mapped to targeting sequences of guides across 8 CRISPR libraries show cell lines of African ancestry have more guides affected than other cell lines. A second histogram of three corrected libraries shows the differences reduced in two cases and both peaks overlapped in the third.
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Stabilizing gRNA Modifications

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