Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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Every student about to graduate shares the same nightmare: the question “So, what comes next?” For those finishing up an undergraduate education or ending a Master’s program, the answer might be to continue in academia and pursue a PhD. However, if you’re anything like me, the ...
Every year we celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources! Ready to sing along with this year's lineup? Right! We're ready to, so let's get started!
Taking the road less traveled and generating a knock-in cell line instead of a knock-out? We’ve got you covered in this blog, with tips and tricks for harnessing the homology-directed repair pathway, designing the best donor DNA, and avoiding common mishaps in this class of ...
Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant protein that will be produced. Many ...
This Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some are written by Addgenies and some are written by the depositors themselves. All of them have cool new tools, techniques, or information that is now available for other ...
Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social media and blog to say thank you to all the many scientists and researchers who deposit with us!
We are all oh so familiar with the nuclease Cas9, but how does it stack up to the new Cas enzymes on the block? The Cas family of proteins has many useful genome engineering enzymes, each with their own unique features and quirks. Here, we will review the genome engineering ...
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