Master plasmid fundamentals, CRISPR techniques, AAV serotype selection, and antibody applications. Written by scientists, for scientists.
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The stress of finding a ‘good’ antibody is something we’ve all experienced. Finding an antibody that works for your application, specifically detects your protein, is species compatible, and doesn’t come with a high background can be a huge challenge. Epitope tags eliminate the ...
The biomedical field is often concerned with understanding the cause of diseases and how to treat those diseases. The “cause of disease” often requires understanding the disease genetics and the “treatment” usually requires drugs. While we often think of these two fields as ...
Around 20% of human genes have no known function or a poorly defined function (Wood, et al). In the microbial world, approximately 50% of predicted genes have unidentified roles as well (Vanni, et al). Is it true that this many genes are truly dispensable or are the current ...
We often think about the brain’s function in terms of its regions. But equally important is the way the brain connects across these regions, a process driven by neurons. By studying how neurons are physically connected, we can begin to understand how the brain works. It’s kind ...
You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research on the two proteins and come up with a great experimental plan that requires indirect staining of both targets in your specimen. You scour the literature and find an ideal ...
Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an incredibly useful transferable skill. Being able to stand up in front of an audience and convey your ideas clearly and strategically is powerful in almost any career. It allows ...
Ever wondered where human embryonic kidney (HEK) cells originated from? Or why they are such a commonly used cell culture model? In this blog we will spill the beans on all things HEK, from the first experiments isolating the cell line all the way to modern applications.
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